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Updated: Sep 11, 2025

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病毒含有的两胞体的相对体积微镜,由光引导的聚焦离子束扫描电子显微镜启用

Miriam Susanna Lucas1, Katarina Wendy Schmidt2, Christian Münz2

  • 1Scientific Center for Optical and Electron Microscopy (ScopeM), ETH Zurich, 8093 Zurich, Switzerland.

STAR protocols
|August 16, 2025
PubMed
概括

本研究提出了一种使用体积相关光和电子显微镜 (vCLEM) 来分析真核细胞中含有病毒的桃体的协议. 该方法允许对罕见的细胞事件进行详细的3D超结构建模.

关键词:
基于细胞的测试免疫学 免疫学 免疫学显微镜的使用方法

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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