消毒剂驱动的选择性基因表达和代谢演变:不稳定的亚酸盐积累和混合型部分脱/亚酸盐系统中增强的anammox活性
Yi Guo1, Jingfeng Gao1, Yi Zhang1
1National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.
Journal of hazardous materials
|August 16, 2025
概括
消毒剂破坏了混合性部分脱/亚纳摩克斯 (MPDA) 系统中的去除,通过改变微生物群落和代谢途径来降低效率. 由于病毒毒性因子增加和抗生素耐药性基因增加,Candidatus Kuenenia成为一种公共卫生问题.
科学领域:
- 环境微生物学环境微生物学
- 污水处理 污水处理 污水处理
- 生物修复是一种生物修复.
背景情况:
- 混合营养的部分脱/anammox (MPDA) 是一个高效的去除过程.
- 消毒剂可以破坏MPDA系统的稳定性,影响去除效率.
研究的目的:
- 调查消毒剂对MPDA系统性能的影响.
- 阐明系统中断背后的微生物和代谢机制.
- 评估微生物转移对公共卫生的影响.
主要方法:
- 在消毒剂压力下分析去除效率.
- 微生物社区结构分析 (基因丰富).
- 代谢途径基因表达分析.
- 细胞外聚合物物质 (EPS) 组成分析.
主要成果:
- 由于化物供应不稳定,去除效率从95%降至83%.
- 部分脱 (PD) 被硫自和异脱活动的转变扰乱,细菌丰度发生变化 (例如,Thauera增加).
- 消毒剂暴露改变了EPS组成 (蛋白质增加,多糖减少),提高了代谢和中央代谢途径基因的调节,并在Candidatus Kuenenia中诱导了抗生素耐药性基因 (ARG) 和毒性因子 (VF).
结论:
- 消毒剂通过改变微生物群落和代谢功能来破坏MPDA系统,同时促进适应性抵抗机制.
- 候选菌 (Candidatus Kuenenia) 是VF和ARG的重要贡献者,构成潜在的公共卫生风险.
- 了解这些影响对于优化废水处理弹性和安全至关重要.
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