相关实验视频

Updated: Sep 11, 2025

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Published on: October 30, 2013

来自CALD1的circ-0003746针对miR-526b促进EMT介导的膀癌进展

Dengke Yang¹, Weipu Mao², Lei Jiang³

¹Tongji University School of Medicine, Shanghai, China; Department of Urology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Experimental cell research

|August 16, 2025

概括

循环RNAcirc-0003746通过调节miR-526b/表皮-介质细胞过渡轴来促进膀癌 (BCa) 的进展. 沉默circ-0003746抑制BCa细胞生长和迁移,表明其作为BCa生物标志物的潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

CALD1基因在膀癌 (BCa) 的发展中起着至关重要的作用.
循环RNA (circRNA) circ-0003746,来自CALD1,在BCa中有一个不完全理解的功能.

研究的目的:

研究circ-0003746在膀癌进展中的功能作用.
阐明circ-0003746参与BCa.的分子机制.

主要方法:

定量实时PCR评估BCa组织和细胞系中的circ-0003746表达.
在体外和体内实验中评估circ-0003746沉默对BCa细胞增殖和迁移的影响.
路西法酶记者测定证实circ-0003746和miR-526b之间的相互作用.

主要成果:

与正常对照组相比,Circ-0003746在BCa组织和细胞系中显著过度表达.
沉默circ-0003746有效地抑制了BCa细胞的增殖和迁移在体外和体内.
miR-526b被确定为circ-0003746的直接标,它们的相互作用通过光酶试验得到证实.
发现Circ-0003746通过海绵miR-526b促进上皮层-介质细胞过渡 (EMT),从而推动BCa的进展.

结论:

Circ-0003746通过调节miR-526b/EMT通路,在膀癌中起到致癌作用.
Circ-0003746代表了潜在的诊断生物标志物和膀癌的治疗点.

关键词:

膀癌是一种癌症.在CALD1中使用.周围的-000374646 的情况.在EMT中,EMT是EMT.这就是miR-526bb.

更多相关视频

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Published on: January 5, 2018

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Published on: September 13, 2018

相关概念视频

Metastasis

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...