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蛋白质糖化修饰作为一种新的法医生物标志物,用于区分单胞胎双胞胎

Yun-Zhou Chen¹, Yang Li², Yi-Long Wang³

¹School of Forensic Medicine, China Medical University, Shenyang 110122, PR China.

Forensic science international

|August 17, 2025

概括

蛋白质糖化模式可以可靠地区分单胞胎 (MZ) 双胞胎,克服基于DNA的法医识别的局限性. 这项研究强调了甘化作为个体特异性的新生物标志物.

科学领域:

法医科学法医科学法医科学
生物化学生物化学
蛋白质组学是指蛋白质组学.

背景情况:

鉴别单胞胎 (MZ) 双胞胎对于法医科学来说是具有挑战性的,因为它们具有相同的DNA配置文件.
传统的短串重复 (STR) 打字对于MZ双胞胎歧视是无效的.
现有的MZ双胞胎分化表观遗传标记通常缺乏稳定性,不适合法医样本.

研究的目的:

研究蛋白质糖化修饰作为一种新且稳定的生物标志物,用于区分MZ双胞胎.
在外周血液中探索N-糖化和O-糖化模式,用于法医识别.
解决MZ双胞胎歧视中可靠生物标志物的需求,超越DNA分析.

主要方法:

使用液体染色学-双重质谱法 (LC-MS/MS) 和西斑 (WB) 分析N-甘化和O-甘化模式.
对三对MZ双胞胎和六个无关联个体的外周血液样本的调查.
随机将双胞胎对分为A组和B组进行比较分析.

主要成果:

在MZ双胞胎中,LC-MS/MS确定了720种独特的N-甘化改性,其中77种是A组独有的,50种是B组独有的.
基于O-GlcNAc表达模式,WB分析成功地区分了无关个体和MZ双胞胎.
蛋白质甘氨基化修饰表明了作为MZ双胞胎分化可靠生物标志物的潜力.

结论:

蛋白质糖化修饰在法医科学中为区分MZ双胞胎提供了一个有希望和稳定的方法.
这项研究通过引入新的基于糖化酶的生物标志物,推进了法医识别能力.
需要进一步验证才能在法医应用中得到广泛采用,并在个性化医学中进行探索.

关键词:

法医识别识别法医识别双胞胎是单胞胎的双胞胎.在N-glycosylation的过程中.O-GlcNAc 修饰的改变蛋白质的糖化化是蛋白质的糖化.

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