相关实验视频

Updated: Sep 11, 2025

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

Published on: December 19, 2020

一种昆虫β1-3-galactosyltransferase能够有效合成多位点T抗原糖联合物

Yongheng Rong¹, Weian Mao¹, Mei Wang¹

¹National Glycoengineering Research Center, Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.

Bioorganic chemistry

|August 17, 2025

概括

研究人员特征了一种新型酶,DmC1GalT1,用于合成瘤相关碳水化合物抗原 (TACAs). 这种酶提供了一种更有效,更可持续的方法来生产用于癌症研究和治疗开发的关键甘油合物.

科学领域:

葡萄糖科学 (Glycoscience) 是一种科学.
酶学是一种酶学.
生物医学研究的研究.

背景情况:

瘤相关碳水化合物抗原 (TACA),特别是T抗原 (Galβ1-3GalNAc),在人类癌症中很普遍.
在体外酶合成Galβ1-3GalNAc结构在糖生物学中是一个重大挑战.

研究的目的:

为了描述一种新的β1-3-galactosyltransferase,DmC1GalT1来自D. melanogaster.
评估其对高效和可持续合成Galβ1-3GalNAc结构和相关的糖结合物的潜力.

主要方法:

DmC1GalT1的酶表征,包括温度和pH的耐受性.
对催化效率和基质特异性的评估 (UDP-Gal和UDP-GalNH2).
DmC1GalT1在合成各种葡萄糖结合物 (多糖,糖,糖蛋白) 中的应用.

主要成果:

DmC1GalT1表现出广泛的温度 (16-45°C) 和pH (5.0-8.5) 的耐受性.
该酶的催化效率比CjCgtBΔ30高16倍,并防止产品过度修改.
通过使用DmC1GalT1成功合成了多种不同的糖结合物,其中UDP-Gal.T1的产量很高.

结论:

DmC1GalT1提供了一个扩展和高效的酶途径来合成Galβ1-3GalNAc结构.
这种酶对未来在糖科学和生物医学中的应用具有显著的潜力,可实现可持续的糖结合物合成.

关键词:

我们的C1GalT1T1 酶合成酶的合成这是一种O-糖基化.这是一种T抗原.β13-银酸盐转移酶是什么

更多相关视频

The MultiBac Protein Complex Production Platform at the EMBL

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Published on: February 5, 2018

相关概念视频

Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

Protein Glycosylation

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...

Proteoglycans

Proteoglycans

Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...