通过使用抗溶剂沉,有效地从石法兰的污中净化CrocinI,对食品技术的影响
Elmira Fotouhi1, Fateme Mirzajani2,3, Hassan Rezadoost4
1Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, P.O. Box 19835-389, Evin, Tehran, Iran.
Scientific reports
|August 17, 2025
概括
抗溶剂沉有效地集中了沙夫兰中有价值的素化合物,提高了纯度和产量. 这种具有成本效益的方法改善了关键的生物活性成分crocin-I的提取,使得沙夫兰净化更容易获得.
科学领域:
- 植物化学 植物化学
- 自然产品化学 自然产品化学
- 过程化学 过程化学
背景情况:
- 沙夫兰含有克罗辛化合物,是一种强大的抗氧化剂,具有抗炎和抗癌特性.
- 克罗辛的高商业价值受制于大规模,高纯度提取的挑战.
- 克罗辛-I,一种特定的克罗辛,对温度敏感,造成净化困难.
研究的目的:
- 为了选择性地提高沙夫兰提取物中总克罗辛和克罗辛-I的度.
- 使用响应表面方法 (RSM) 来优化抗溶剂沉.
- 开发一种具有成本效益和可扩展的crocins净化方法.
主要方法:
- 适用于沙夫兰提取物的抗溶剂沉技术.
- 使用RSM优化参数,包括沙弗朗度,抗溶剂与溶剂比率,添加率和温度.
- 乙烯酸乙酸和乙烯酸乙作为抗溶剂的评估.
主要成果:
- 在优化条件下,使用乙烯酸乙烯的总克罗辛含量增加了81%.
- 克罗辛-I 构成 55% 的总克罗辛与乙烯酸乙烯.
- 乙乙酸乙烯酸增加了crocin-I含量从26%增加到47%,并降低了picrocrocin.
- 该方法避免了复杂的加热/冷却,保存了热敏感化合物.
结论:
- 抗溶剂沉是一种成本效益高,简单,高效的方法,可以从番石榴中丰富crocin.
- 优化的工艺显著提高了纯克罗辛的产量,包括热敏感的克罗辛-I.
- 这种技术为沙夫兰提取和净化挑战提供了可扩展和实用的解决方案.
相关概念视频
01:10Recrystallization: Solid–Solution Equilibria
1.2K
Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...
1.2K
01:12Precipitation Processes
589
The experimental conditions in a gravimetric analysis should be optimized to maximize the particle size and purity of the obtained precipitate. Ideally, the concentration of the precipitating reagent should be low with effective stirring to maintain low relative supersaturation for the growth of large crystals. In homogeneous precipitation, the precipitant is slowly generated by a chemical reaction in the solution to avoid local reagent excesses. For example, urea decomposes gradually to...
589
00:52Washing, Drying, and Ignition of Precipitates
1.1K
After filtration, the precipitate is washed to remove coprecipitated impurities and any remaining mother liquor. Colloidal precipitates, such as silver chloride, are washed with an electrolyte (such as dilute nitric acid) to prevent the peptization of the precipitate. In the case of slightly soluble precipitates, the wash solution contains a common ion to reduce solubility. Lead sulfate, which is slightly soluble in water, is washed with dilute sulfuric acid. Similarly, wash solutions may be...
1.1K
01:17Precipitation and Co-precipitation
2.0K
Precipitation and coprecipitation methods can be used to separate a mixture of ions in a solution. In qualitative inorganic analysis, ions that form sparingly soluble precipitates with the same reagent are separated based on the differences in solubility products. For example, consider the separation of Cu(II) and Fe(II) ions by precipitation as insoluble sulfides. First, copper(II) sulfide is precipitated by the addition of acidic H2S, where the dissociation of H2S is suppressed. Adding H2S...
2.0K
01:09Colloidal precipitates
753
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
753