相关实验视频

Updated: Sep 11, 2025

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Published on: November 13, 2015

内生逆转录病毒的异常复发导致心肌炎和心力衰竭

Junhao Xiong¹, Shasha Zhang¹, Zilong Geng¹

¹Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Department of Cardiovascular Surgery, Shanghai Chest Hospital, Engineering Research Center of Techniques and Instruments for Diagnosis and Treatment of Congenital Heart Disease, Institute of Developmental and Regenerative Medicine, Xin Hua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China (J.X., S.Z., Z.G., J.L., K.C., H.H., Y.W., Y.S., P.Y., Yige Li, S.W., X.C., A.F.C., X.H., R.L., D.Z., K.S., B.Z.).

|August 18, 2025

概括

内源逆转录病毒 (ERV) 在心力衰竭中的复发是由TRIM28耗尽所驱动的. 这种ERV激活会触发先天性免疫路径,导致心肌炎和心力衰竭,但也提供心脏保护,成为治疗点.

科学领域:

基因组学
免疫学
心脏病学

背景情况:

内生逆转录病毒 (ERV) 占人类基因组的8%以上.
异常ERV复发与各种疾病有关.
在心力衰竭 (HF) 中ERV复发的作用仍然未被研究.

研究的目的:

研究ERV复发在心力衰竭中的发生率和作用.
确定心脏中ERV复苏的分子调节器和下游途径.
探索ERV介导的炎症途径的治疗潜力.

主要方法:

在人类和小鼠HF模型中建立了ERV评估的总RNA测序管道.
为研究ERV复苏调节剂 (TRIM28,SETDB1) 生成了心肌特异性小鼠线.
用于TRIM28过度表达的腺相关病毒9型和测试的TLR7/9抑制剂.

主要成果:

在HF模型中观察到ERV的突出激活,特别是I类.
通过表观遗传变化 (H3K9me3,m6A) 导致了ERV激活.
激活ERV触发了TLR7/9-NF-κB通路,导致心肌炎和HF;治疗干预改善了心脏功能.

结论:

ERV复苏是HF的一个关键特征,由心肌细胞TRIM28耗尽驱动.
通过ERV-TLR7/9-NF-κB途径诱导心肌炎和心肺炎,同时也提供心脏保护.
针对TRIM28-ERV-TLR7/9-NF-κB轴为心肌炎和心血管炎提供治疗策略.

关键词:

在 NF-κB 一个TIM28 内生逆转录病毒心脏衰竭心肌炎

更多相关视频

Gene Transfer for Ischemic Heart Failure in a Preclinical Model

Gene Transfer for Ischemic Heart Failure in a Preclinical Model

Published on: May 15, 2011

Noninvasive Assessment of Cardiac Abnormalities in Experimental Autoimmune Myocarditis by Magnetic Resonance Microscopy Imaging in the Mouse

Noninvasive Assessment of Cardiac Abnormalities in Experimental Autoimmune Myocarditis by Magnetic Resonance Microscopy Imaging in the Mouse

Published on: June 20, 2014

相关概念视频

Myocarditis I: Introduction

Myocarditis I: Introduction

Myocarditis is inflammation of the myocardium, which is the muscular layer of the heart.EtiologyMyocarditis has a diverse etiology, including a wide range of infectious and non-infectious causes:Infectious CausesViral: Common viruses include Coxsackie A and B, adenovirus, parvovirus B19, enteroviruses, and influenza A.Bacterial: Examples include infections caused by Streptococcus, Staphylococcus, and Mycoplasma species.Rickettsial: Infections like Rocky Mountain spotted fever can result in...

Myocarditis III: Medical Management

Myocarditis III: Medical Management

Myocarditis: Comprehensive Medical ManagementMyocarditis, the heart muscle inflammation, requires a comprehensive medical management strategy that addresses the underlying cause, provides supportive care, manages symptoms, and reduces cardiac workload.Infections and Autoimmune CausesAdminister appropriate antimicrobial therapy when an infectious agent causes myocarditis. For instance, penicillin treats infections caused by Group A Streptococcus. In cases where autoimmune processes are...

Myocarditis II: Clinical Features and Diagnostic Tests

Myocarditis II: Clinical Features and Diagnostic Tests

Myocarditis is an inflammation of the heart muscle. The symptoms vary widely, encompassing asymptomatic presentations to severe, acute manifestations.Clinical PresentationAsymptomatic cases: In some instances, myocarditis may be asymptomatic, with the infection resolving without intervention. These cases often go undetected unless discovered incidentally through diagnostic imaging or tests conducted for other reasons.General Early Symptoms: Early symptoms of myocarditis are non-specific and can...

Endocarditis I: Introduction

Endocarditis I: Introduction

Introduction:Endocarditis is the infection of the endocardium, the inner lining of the heart and its valves. When the heart muscle is involved, the condition is termed myocarditis, while an infection of the outer lining is called pericarditis. Infective endocarditis (IE) primarily affects the endocardium, where pathogens adhere to the valves or lining, forming vegetation that can lead to severe complications. Infective endocarditis occurs when microorganisms, usually bacteria from other body...

Rheumatic Heart Disease I: Introduction

Rheumatic Heart Disease I: Introduction

Rheumatic heart disease or RHD is a chronic condition that results from rheumatic fever, causing permanent damage to the heart valves.Etiology and Risk FactorsIt primarily arises from rheumatic fever, an inflammatory disease that can develop after untreated or inadequately treated group A streptococcal (GAS) pharyngitis. Streptococcus spreads through direct contact with oral or respiratory secretions. While the bacteria are the causative agents, factors like malnutrition, overcrowding, poor...

Endocarditis II: Clinical Features of Infective Endocarditis

Endocarditis II: Clinical Features of Infective Endocarditis

Endocarditis can present various clinical features depending on the causative organism and the patient's underlying health conditions. Initially, the clinical features of infective endocarditis develop gradually, presenting with nonspecific symptoms that can be easily mistaken for other illnesses.General SymptomsEarly symptoms of infective endocarditis are fever, chills, weakness, malaise, fatigue, and weight loss. These symptoms reflect the systemic nature of the infection and the body's...