在Saccharomyces cerevisiae中开发一种高效的酸响应生物传感器
Xueqing Pang1, Jingyuan Zhu1, Yuqing Li1
1College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, PR China.
Synthetic and systems biotechnology
|August 18, 2025
概括
在酵母中开发了一种新的生物传感器,以控制p-酸的产生,这是制造有价值化合物的关键步骤. 这种工具有助于优化生物合成途径,以增加自然产品的产量.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 微生物生物技术 微生物生物技术
背景情况:
- 生物传感器对于监测和调节细胞内生物合成途径至关重要.
- 在微生物细胞工厂中提高自然产品产量需要精确控制代谢途径.
- 酸是合成多和黄的重要前体.
研究的目的:
- 在Saccharomyces cerevisiae中开发一种新型的生物传感器,对p-coumaric酸产生反应.
- 为严格监管和增强活动设计混合促进者.
- 通过战略蛋白质工程和促进体选择来优化生物传感器性能.
主要方法:
- 使用来自 Bacillus subtilis 的 BsPadR 抑制剂构建生物传感器.
- 混合促进器的工程 (例如,P_BS1-CCW12,P_BST1,P_ERG9) 用于BSPadR监管.
- 将SV40核定位信号 (NLS) 融合到BsPadR以增强功能.
- 动态调节CrtE (geranylgeranyl pyrophosphate synthase) 的作用,用于烯生物合成.
主要成果:
- 混合P_BS1-CCW12促进剂显示了紧密的BsPadR调节,并增加了与p-coumaric酸的活性.
- 通过使用较弱的促进剂 (P_BST1,P_ERG9) 来缓解过度BsPadR导致的生长抑制.
- C端 SV40-NLS 融合增强了 BsPadR 生物传感器的性能.
- 在CrtE的动态调节中成功应用,用于利科生物合成.
结论:
- 在S. cerevisiae中成功开发了一种新型的p-酸响应生物传感器.
- 生物传感器可以动态调节生物合成途径,促进高通量选.
- 这种工具有助于优化微生物细胞工厂中的p-酸衍生物生产.
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