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在宫癌患者中差异表达的lncRNA和mRNA的生物信息学分析

Xiaohua An^1,2, Xiaoxue Huang¹, Qiujie Yu^1,2

¹Department of Laboratory Medicine, The Third Affiliated Hospital of Zunyi Medical University (The First People's Hospital of Zunyi), Zunyi, Guizhou, China.

Frontiers in bioinformatics

|August 18, 2025

概括

这项研究确定了宫癌中差异表达的长非编码RNA (lncRNA) 和信使RNA (mRNA),揭示了它们与HPV16感染的关联以及作为诊断标记物的潜力. 像CDKN2B-AS1这样的关键lncRNA与HPV16 E6/E7表达有关.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

子宫癌是一个重大的全球健康问题,通常是由人类乳头瘤病毒 (HPV) 感染引起的.
了解长非编码RNAs (lncRNAs) 和信使RNAs (mRNAs) 在与HPV相关的宫癌中的作用,对于开发新的诊断和治疗策略至关重要.

研究的目的:

研究宫癌组织中lncRNAs和mRNAs的表达特征,特别是在与HPV16相关的病例中.
确定这些分子的临床意义,阐明它们的生物功能.
在HPV感染的背景下探索lncRNAs和mRNAs之间的调节关系.

主要方法:

高通量测序用于分析宫癌和相邻正常组织中的lncRNA和mRNA表达.
基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析进行了对差异表达mRNA的功能注释.
蛋白与蛋白相互作用 (PPI) 网络是为了理解分子相互作用而构建的.
用RT-qPCR和GEPIA数据库分析进行验证.
为了研究病毒蛋白对lncRNA表达的影响,建立了一种过度表达HPV16 E6/E7的稳定细胞系.

主要成果:

发现大量的lncRNAs (3,608个上调,4,383个下调) 和mRNAs (3,666个上调,2,220个下调) 在宫癌组织中表达不同.
功能性丰富分析表明,差异表达的mRNAs在细胞周期和衰老中具有重要作用,以及像cAMP和MAPK这样的途径.
鉴定出了20个反意义 lncRNAs,它们可能调节它们相应的mRNAs.
RT-qPCR证实CDKN2B-AS1,HAGLROS和GATA6-AS1可能受到HPV16 E6/E7.7的调节.

结论:

这项研究提供了对HPV16相关的宫癌中lncRNA和mRNA表达的全面分析.
鉴定出差异表达的lncRNA和mRNA具有作为诊断生物标志物的潜力.
这些发现突出了宫癌发生的复杂分子机制,以及HPV在调节lncRNA表达中的作用.

关键词:

在HPV16 E6/E7E7的病例中.宫癌:子宫癌是一种癌症.高通量测序的高通量测序在 lncRNA 中,它们是mRNARNA.

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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...