相关实验视频

Updated: Sep 11, 2025

Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release

Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release

Published on: October 6, 2018

NGF刺激改变了背部根结节神经元的轴突中的转录组和表面TrkB表达

Maximilian Koch¹, Manas Kshirsagar¹, Ankita Rawat¹

¹Institute of Clinical Neurobiology, University Hospital Würzburg, Würzburg, Germany.

Neurobiology of pain (Cambridge, Mass.)

|August 18, 2025

概括

神经生长因子 (NGF) 触发介素-7 (IL-7) 转录在感觉神经元轴突中增加,可能导致疼痛. 远端神经元区域可以自主调节对NGF的反应.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

神经生长因子 (NGF) 在神经元受伤后释放,启动炎症反应.
NGF影响神经元基因表达和亚细胞反应,可能导致疼痛.
由NGF进行的轴突转录基因调制与疼痛的开始和维持有关.

研究的目的:

研究由NGF刺激的感觉神经元中的急性转录和分子调制.
探索NGF在调节轴突基因表达和蛋白质受体动态中的作用.

主要方法:

在微流体腔室中培养背部根结质神经元.
使用NGF刺激神经元轴突,观察细胞反应.
分析转录水平,蛋白质受体分布和核糖体子单元组织.

主要成果:

NGF刺激增加了互白素-7 (IL-7) 转录的轴突水平,导致IL-7的释放.
在NGF刺激后的感觉神经元生长中观察到核糖体子单元 (60S和40S) 的重组.
热氨酸激酶B (TrkB) 受体在生长的血膜上的动态再分配,从内质网膜 (ER) 招募.

结论:

轴突IL-7转录的上调和释放表明它在NGF介导的神经元反应中起作用.
在感官神经元生长中,NGF诱导局部的核糖体重组和TrkB受体贩运.
感觉神经元中的远端细胞结合机制可能会自主调节信号传递和翻译,以响应NGF.

关键词:

轴突转录基因组的转录基因组一个DRG神经元.介质蛋白是介质蛋白中的一种.神经生长因子的神经生长因子

更多相关视频

Use of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure

Use of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure

Published on: August 27, 2019

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Published on: August 17, 2012

相关概念视频

Enzyme-linked Receptors

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...