Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Microarrays02:34

DNA Microarrays

18.4K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
18.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Phase Engineering of Iridium Oxides Enables Direct Coupling of Proton Exchange Membrane Water Electrolysis With Intermittent Electrical Energy.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Corrigendum to "Xanthoxylin alleviates dextran sulfate sodium (DSS)-induced colitis by targeting macrophage infiltration via the tumor necrosis factor (TNF)/nuclear factor-kappa B (NF-κB) signaling pathway" [Phytomedicine 153:157971 (2026) PMID: 41720014].

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

AdaptCMVC++: Robust and Flexible Adaptation to Incremental Views in Continual Multi-view Clustering.

IEEE transactions on pattern analysis and machine intelligence·2026
Same author

Rapid and real-time detection of trace BPA based on solution-gated graphene field-effect transistor by cerium-doped ZIF-8 carbon material.

Talanta·2026
Same author

A Multi-Regional Single-nucleus Atlas of the Huntington's Disease Brain.

Scientific data·2026
Same author

Machine learning-aided 3D-AFM for identification of spatial heterogeneity in interfacial solvation structures.

Nanoscale·2026

相关实验视频

Updated: Sep 11, 2025

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.4K

分子编码珠子用于多重复核酸定量检测.

Wen Cheng1, Wanyan Zeng1, Jiahao Fan1

  • 1State Key Laboratory of Chemo and Biosensing, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, P. R. China.

Analytical chemistry
|August 18, 2025
PubMed
概括

这项研究增强了用于多重核酸检测的DNA编码,改进了探针设计,并引入了用于精确量化的新染料. 这种先进的平台可以同时对八种核酸进行快速,高吞吐量分析.

更多相关视频

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.4K
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.4K

相关实验视频

Last Updated: Sep 11, 2025

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.4K
Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.4K
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.4K

科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 分析化学 分析化学

背景情况:

  • 以前的DNA编码方法在定量检测和多重复合能力方面遇到了局限性.
  • 对多个核酸的准确和有效量化对于各种生物和诊断应用至关重要.

研究的目的:

  • 提出一种改进的DNA编码方法,具有增强的定量检测能力.
  • 为快速,稳固和高通量多重核酸分析开发一个多功能平台.

主要方法:

  • 整合了三个关键的修改:改变了编码探头中的杂交区域,改变了珠子类型,并添加了第三种光染料.
  • 开发了一种三光体系统,用于同时量化八种不同的核酸.
  • 利用流细胞计与简化的两刺激和三排放通道系统.

主要成果:

  • 在皮科莫拉 (pM) 水平达到检测极限,满足多重分析的灵敏度要求.
  • 证明了同时编码和目标检测,简化了工作流.
  • 通过使用开发的系统,同时成功量化了八种不同的核酸.

结论:

  • 改进的DNA编码方法为定量核酸检测提供了一个多功能和高效的平台.
  • 这种plug-and-play策略显著推进了多重核酸分析,使高速和强大的高吞吐量应用成为可能.