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在室温下便样本中稳定人类RNA和DNA
Regina Preywisch1, Roger Løvlie1, Moritz Eidens1
1Mainz Biomed Germany GmbH, Mainz, Germany.
BioTechniques
|August 18, 2025
概括
人类mRNA和DNA在便样本中稳定长达15天,使用新的稳定缓冲器. 这有助于改善非侵入性胃肠道疾病检测和生物标志物发现.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 胃肠病学 胃肠病学
背景情况:
- 便样本提供了一种非侵入性方法来诊断肠胃疾病,如结肠直肠癌.
- 便中信使RNA (mRNA) 的不稳定性对准确的诊断测试构成了挑战.
- 开发可靠的方法来保存便中的核酸对于推进诊断至关重要.
研究的目的:
- 在便样本中评估人类mRNA和DNA的稳定性,这些便样本经过专门的稳定溶液处理.
- 评估室温储存15天对核酸完整性的影响.
- 增强存储协议,使基于mRNA的诊断和生物标志物发现更加可靠.
主要方法:
- 收集了97个便样本,并用稳定溶液处理它们.
- 在室温下储存样本,分析1日和15日的核酸稳定性.
- 量化了总核酸产量,并评估了三个特定mRNA标记物的稳定性:CEACAM5,PTGS2和CTTN.
主要成果:
- 核酸总产量从第1天 (平均112 ng/μL) 增加到第15天 (平均165 ng/μL),因为同质化和溶解的改善.
- 人类DNA在14天的储存期内表现出稳定性.
- CEACAM5和PTGS2mRNA标记保持稳定,而CTTN显示表达量有统计学意义但略有下降 (p < 0.0001).
结论:
- 稳定缓冲器有效地保留在室温下储存的便样本中的DNA和mRNA,长达15天.
- 这些发现支持使用这种稳定方法来提高非侵入性胃肠道疾病诊断的可靠性.
- 这项研究通过从便样本中更准确地发现生物标志物,为个性化医学做出了贡献.
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