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大型动物3D成像组织光学清除技术的进步

Yating Deng^1,2, Jianyi Xu^1,2, Tingting Yu^1,2

¹MOE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Advanced Biomedical Imaging Facility, Huazhong University of Science and Technology, Wuhan, 430074, China.

Frontiers of optoelectronics

|August 18, 2025

概括

组织光学清除使大型动物的高分辨率3D成像成为可能,这对生物医学研究至关重要. 本次审查将动物和大型动物协议结合起来,解决先进研究应用的特定物种挑战.

科学领域:

生物医学工程生物医学工程
神经科学是一个神经科学.
历史学历史学历史学

背景情况:

组织架构的三维 (3D) 重建对于生物医学研究至关重要.
组织光学清除克服光散射,使高分辨率的3D成像.
大型动物模型 (猪,非人类灵长类动物) 紧密模仿人类解剖学和生理学,使它们对研究有价值.

研究的目的:

系统地审查组织光学清除方法从动物转化到大型动物的转化.
识别和解决清除大型动物组织的特定物种挑战.
总结当前的应用,并为大型动物生物医学研究提出优化策略.

主要方法:

对现有关于组织光学清除协议的文献进行系统审查.
对大脑结构和器官组成影响清除效率的特定物种差异的分析.
在大型动物模型中最近应用的概述.

主要成果:

已建立的动物协议面临挑战,当扩展到大型动物,由于尺寸和组成的变化.
大脑和器官组成的特定物种差异严重影响清除效率.
在大动物的神经电路映射和感官器官成像中展示了成功的应用.

结论:

从动物到大型动物的方法转换需要解决跨物种兼容性的障碍.
建议采用优化策略,以增强大型动物模型中的组织光学清除.
本综述作为推进使用光学清除技术的大型动物生物医学研究的参考.

关键词:

生物组织生物组织.大型动物大型动物光学成像技术的使用.组织的光学清除.

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