Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Microtubules in Signaling

Microtubules in Signaling

The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Systemic Immune-Inflammatory Index For Evaluating Robotic and Laparoscopic Proximal Gastrectomy in Upper Gastric Cancer.

Journal of visualized experiments : JoVE·2026

Same author

Development and validation of a nomogram model for mortality risk in burn patients: Triglyceride-glucose index as an independent novel prognostic marker.

Burns : journal of the International Society for Burn Injuries·2026

Same author

Dynamic multimodal radiomic model for survival prediction in cervical cancer: a multi-cohort study.

NPJ precision oncology·2026

Same author

[Deep Learning-Based Automatic Segmentation Model for Radiotherapy Target Delineation of Brain Metastases and Its Clinical Applications].

Zhongguo yi liao qi xie za zhi = Chinese journal of medical instrumentation·2026

Same author

Structurally-Informed 3D Gaussian Splatting for Limited-Angle CBCT.

IEEE transactions on medical imaging·2026

Same author

Lactate metabolism and lactylation in cancer: from pathogenesis to therapeutic advances.

Signal transduction and targeted therapy·2026

Search research articles

相关实验视频

Updated: May 4, 2026

A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies

A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies

Published on: September 23, 2014

基于的组织微阵列构造用于瘤和疾病研究.

Junwen Cai¹, Ying Zheng², Wenguang He³

¹Department of Medical Laboratory, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University.

Journal of visualized experiments : JoVE

|August 18, 2025

概括

一种新的基于合物的方法增强了用于生物标志物研究的组织微阵列 (TMA) 构造. 这种技术改善了组织的固定和稳定性,为中等吞吐量研究提供了可靠,具有成本效益的替代方案.

更多相关视频

SpOT the Correct Tissue Every Time in Multi-tissue Blocks

SpOT the Correct Tissue Every Time in Multi-tissue Blocks

Published on: May 31, 2015

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

相关实验视频

Last Updated: May 4, 2026

A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies

A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies

Published on: September 23, 2014

SpOT the Correct Tissue Every Time in Multi-tissue Blocks

SpOT the Correct Tissue Every Time in Multi-tissue Blocks

Published on: May 31, 2015

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

科学领域:

生物医学工程生物医学工程
病理学病理学病理学
分子生物学分子生物学

背景情况:

组织微阵列 (TMA) 技术使组织样本用于疾病生物标志物研究的高通量分析成为可能.
传统的TMA施工方法带来了挑战,包括复杂性,时间消耗和可变的准确性.
需要改进TMA施工技术,以提高效率和可靠性.

研究的目的:

开发和验证一种新的基于粘合剂的方法,用于组织微阵列的构建.
解决传统TMA方法的局限性,重点是改善组织核心固定和结构稳定性.
通过系统验证来评估基于的TMA方法的性能.

主要方法:

开发一种基于的协议,用于TMA块的制备.
系统验证使用血素和埃 (HE) 染色进行组织学评估.
目标蛋白质的免疫组织化学 (IHC) 分析和光现场混合 (FISH) 分析.

主要成果:

基于合剂的方法显示出出色的切片完整性和提高组织核心的结构稳定性.
在IHC和FISH分析中观察到更好的信号噪声比率.
实现了一致的批量复制性,表明了可靠性.
这种方法被证明是具有成本效益的,适合中等通量TMA生产.

结论:

基于的TMA构造方法为生物标志物研究提供了可靠且具有成本效益的解决方案.
这种技术克服了传统方法的局限性,提供了改善的组织固定和稳定性.
这种方法对于优先考虑灵活性和样本多样性的研究环境特别有价值,扩大了TMA在学术和诊断环境中的实用性.