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隔离,培养和表征前列腺癌相关的纤维细胞

Somayeh Mirzaaghaei¹, Lidia Avalle², Chiara Verrengia¹

¹Department of Molecular Biotechnology and Health Science, University of Torino.

Journal of visualized experiments : JoVE

|August 18, 2025

概括

这项研究引入了一种方法,从前列腺癌患者中分离癌症相关纤维细胞 (CAF) 和正常纤维细胞 (NF). 这些孤立的细胞使得研究纤维细胞在癌症进展中的作用和新疗法的开发成为可能.

科学领域:

在瘤学瘤学.
细胞生物学细胞生物学
癌症研究癌症研究

背景情况:

瘤流层积极影响瘤的进展,与癌症相关的纤维细胞 (CAF) 是瘤微环境 (TME) 中的关键参与者.
了解CAF与瘤细胞的相互作用对于开发向癌症疗法至关重要.
需要强大的方法来隔离和描述CAF.

研究的目的:

介绍一种将纤维细胞从前列腺癌 (PCa) 样本中分离出来的方法.
为了使同一个患者的CAF和正常纤维细胞 (NFs) 同时分离,进行配对分析.
为维护,永久化和功能测试CAF和NF提供协议.

主要方法:

从瘤和邻近的正常组织中分离纤维细胞的急性前列腺切除试样.
对于初级细胞培养,不朽化和下游体外分析的协议.
功能性测定包括条件介质 (CM) 处理和直接共同培养,以评估CAF/NF对癌细胞增殖,迁移和结独立生长的影响.

主要成果:

建立了一个配对的实验系统,用于比较同一个体的CAF和NF.
提供了细胞维护和功能测试的详细方案.
这些方法有助于研究CAF与瘤细胞的相互作用,并确定治疗点.

结论:

开发的工具包可以全面调查前列腺癌和潜在的其他癌症中的CAF和瘤细胞.
这些相互作用的分子表征可以揭示CAF驱动型瘤发生的关键媒介.
这项研究为新的CAF向癌症治疗奠定了基础.

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