在Diaphorina citri中的内共生皮科尼亚样病毒的功能特征
Shuai Wang1, Muhammad Younas1, Chengtao Bao1
1State Key Laboratory of Agricultural and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
Virus research
|August 18, 2025
概括
类似于Diaphorina citri picorna的病毒 (DcPLV) 在亚洲类植物中广泛存在,垂直和水平传播. DcPLV增强了类绿化细菌 (CLas) 的获取,增加了载体能力.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 植物病理学 植物病理学
- 病毒学 病毒学
背景情况:
- 黄龙 (HLB) 是一种由Candidatus Liberibacter asiaticus (CLas) 引起的破坏性类病.
- 亚洲类植物 (Diaphorina citri) 是CLas.的主要载体.
- D. citri中的内共生病毒,如Diaphorina citri picorna-like virus (DcPLV),正在引起人们的注意,但它们的流行病学和作用尚不清楚.
研究的目的:
- 调查中国D. citri种群中DcPLV的现场流行,传播和生物影响.
- 确定DcPLV在CLas.传播中的作用.
- 为了阐明DcPLV,D. citri和CLas.之间的相互作用.
主要方法:
- 来自中国17个地区的D. citri现场采集 (2023-2024年).
- 使用RT-qPCR检测和量化DcPLV.
- 对DcPLV感染率的时间监测.
- 对垂直和水平传输模式的分析.
- 评估DcPLV对虫繁殖和寿命的影响.
- 病毒局部化和积累的组织特异性分析.
主要成果:
- DcPLV的分布很广泛,平均感染率为35.18%.
- 全年保持高感染水平,观察到垂直和水平传播.
- DcPLV并没有显著影响虫繁殖或寿命.
- 病毒在中肠和唾液腺中积累,随着的发育而增加.
- 在D. citri. 中,DcPLV感染显著增强了CLas的获取和积累.
结论:
- DcPLV是一种常见的内共生病毒,在亚洲类植物中具有一致的流行率.
- 病毒通过垂直和水平途径有效传播.
- DcPLV增强了D. citri对CLas的载体能力,可能会加剧HLB的传播.
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