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预先烧烤的微粒子在中失去其传染性

Orawan Thepmanee¹, Krittapron Keawpanya¹, Chanadda Kasamechotchung²

¹Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand.

Journal of invertebrate pathology

|August 18, 2025

概括

使用KHP或NaHP预发芽的EHP子使它们对无感染力. 这一发现为控制水产养殖中的肝胰腺微分泌症 (HPM) 提供了潜在的策略.

科学领域:

水产养殖是水产养殖的一种方式.
传染性疾病传染性疾病
海洋生物学海洋生物学

背景情况:

养殖面临着由Enterocytozoon hepatopenaei (EHP) 引起的肝胰腺微粒症 (HPM) 的挑战.
EHP感染导致种群的生长迟缓和尺寸变化.
EHP子通过发芽并通过极管入侵肝胰腺细胞来感染.

研究的目的:

调查是否预发芽的EHP子降低了它们在中的传染性.
评估甲酸 (KHP),甲酸 (NaHP) 和素B在诱导EHP子发芽和传染性丧失方面的疗效.
建立一种实验室测定方法,用于评估降低EHP子传染性的化合物.

主要方法:

在试验室中使用KHP (50mM),NaHP (100mM) 或素B (2%) 进行了EHP子的预发芽.
预先发芽的子被用于在受控的水族馆环境中浸泡天真的Penaeus vannamei (PL12阶段).
在14天后,使用嵌套PCR和肝胰腺组织组织染色来评估感染水平.

主要成果:

没有任何暴露于预发芽的EHP子的鱼显示出感染迹象.
暴露于非发芽的EHP子的对照组呈现出60%的感染率.
组织学分析证实,在预先发芽的子组中没有EPH,在对照组中存在EPH.

结论:

在实验室条件下,EHP子的预发芽有效地中和了它们的传染性.
作为潜在的抗EHP药物,KHP和NaHP显示出有前途的作用,因此需要在农场环境中进行进一步的调查.
开发的体内测试可用于选抑制EHP子感染性的新型化合物.

关键词:

在EHP中使用EHP.细胞核虫肝脏病的发生.肝胰腺微型杆菌病.佩内乌斯 (Penaeus vannamei) 是一个水族群.子的发芽子的发芽

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