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Updated: Sep 11, 2025

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
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通过主要编辑策略精确纠正iPSC中的G6PD维扬突变

Chaiwat Netsawang1, Malinee Tongbaen1, Natee Jearawiriyapaisarn2

  • 1Programme in Clinical Hematology Sciences, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.

Scientific reports
|August 18, 2025
PubMed
概括

首次编辑有效地纠正了细胞系中普遍存在的G6PD Viangchan突变. 这种基因编辑工具显示了高精度和最小的副产品,为葡萄糖-6-酸盐脱酶缺乏症提供了一个有前途的治疗策略.

关键词:
克里斯普尔-Cas9是什么意思在G6PD中,G6PD是指G6PD.在IPSC中,IPSC是IPSC.主要编辑主要编辑测序测序是指测序的时间.维安的突变变异.

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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