陆地植物中的光合作用蛋白质的组装依赖的翻译反调节
Rabea Ghandour1,2, Yang Gao1, Stephanie Ruf1
1Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
Nature plants
|August 18, 2025
概括
光合作用复合组合的反调节,称为合成表控制 (CES),在鲁比斯科和光系统II (PSII) 的陆地植物中很普遍,但不是PSI. 这一规定在藻类和陆地植物之间有所不同.
科学领域:
- 植物分子生物学 植物分子生物学
- 光合作用研究研究光合作用.
- 藻类和陆地植物比较基因组学
背景情况:
- 通过合成表现控制 (CES) 调节基于蛋白质复合组合的绿藻中质质编码的光合作用子单元.
- 陆地植物中的鲁比斯科已被描述为CES,但其在其他光合作用复合体中的作用尚不清楚.
研究的目的:
- 调查各种光合作用复合物的陆地植物中CES的存在和程度.
- 为了比较陆地植物 (胚胎) 和绿藻之间的CES机制.
- 在光合作用复合组合中识别保存和分离的调节连接.
主要方法:
- 对具有光系统II (PSII),细胞染色体b6f (Cyt b6f),光系统I (PSI),ATP合成酶,NAD(P) H脱酶样 (NDH) 复合体和Rubisco组装缺陷的陆地植物突变物的系统检查.
- 在翻译层面对反调节的分析.
- 对藻类和胚胎细胞之间的调节联系进行比较分析.
主要成果:
- 在Rubisco中证实了CES,在陆地植物中发现了PSII (psbA,psbB,psbD) 和Cyt b6f (petA,petB) 中的翻译反调节的证据.
- 在ATP合成酶子单元之间观察到潜在的反调节.
- 发现的大多数监管连接在藻类和胚胎细胞之间没有保存;在陆地植物PSI或NDH复合组合中没有发现CES.
- 表明PSII和PSI之间存在监管联系.
结论:
- 陆地植物对PSII和Cyt b6f表现出组装依赖的反调节 (CES),类似于藻类,但在保存的监管联系方面存在显著差异.
- 在陆地植物PSI或NDH复合组装中,CES并不明显,这表明了不同的监管策略.
- 这项研究揭示了植物进化过程中的光合作用复合体组合中反调节的既保守的,又不同的机制.
更多相关视频
08:40Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting
Published on: February 14, 2019
8.4K
08:54Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
Published on: March 20, 2016
9.9K
相关概念视频
01:25Cell Signaling in Plants
5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K
02:00Photoreceptors and Plant Responses to Light
24.5K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
24.5K
01:46The Calvin Benson Cycle
4.7K
Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
4.7K
01:23Regulation of Expression at Multiple Steps
996
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
996
02:24Regulation of Expression Occurs at Multiple Steps
23.3K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
23.3K
01:29Translational Regulation
92
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
92