致病性微生物群通过改变能量代谢来破坏大脑器官的完整结构
Melis Isik1, Cemil Can Eylem2, Kubra Erdogan-Gover3
1Stem Cell Research Lab, Department of Chemistry, Faculty of Science, Ankara University, Ankara, Türkiye.
Molecular psychiatry
|August 18, 2025
概括
病原性细菌通过破坏神经结构和改变细胞代谢来损害大脑器官,可能导致神经退行性疾病. 这项研究强调了细菌对大脑有机生物分子结构的影响.
科学领域:
- 神经科学是一个神经科学.
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 大脑器官 (COs) 为研究人类大脑发育和疾病提供了一个模型.
- 肠-大脑轴和微生物对神经健康的影响是激烈研究的领域.
- 了解微生物与神经组织的相互作用对于神经退行性疾病研究至关重要.
研究的目的:
- 研究致病性 (PM) 和非致病性 (NM) 细菌对大脑器官 (CO) 生物分子结构的影响.
- 在细菌共同培养时分析COs的代谢和转录基因变化.
- 使用代谢流量分析探索细菌与神经细胞的通信.
主要方法:
- COs与非致病性 (NM) 和致病性 (PM) 细菌种群的共同培养.
- 免疫组织化学评估神经元标记物 (TUJ1+).
- 用于转录基因分析的RNA测序 (RNA-seq).
- 蛋白质组分析以确定蛋白质表达变化.
- 基于O稳定同位素标记 (SIL) 的代谢流量分析.
主要成果:
- 暴露于颗粒物减少了TUJ1+神经元,并破坏了CO结构.
- PM改变了CO转录档案,降低了糖解和增加了酸路径活性.
- 蛋白质组分析揭示了与神经疾病相关的PM上调调节蛋白质.
- 细菌共同培养增加了COs中的氧化酸化活性,由TCA循环中介丰富表明.
结论:
- 病原性细菌可以诱导大脑有机生物分子结构和细胞代谢的有害变化.
- 在PM暴露时观察到的COs中的代谢变化与神经退行性疾病途径有关.
- 这项研究建立了一个监测微生物群-脑细胞通信及其代谢后果的平台.
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