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Updated: Sep 10, 2025

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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Published on: November 28, 2017

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没有形态变化的Allostery:一个本地质谱视角.

He Mirabel Sun1, Kacie A Evans1, Morgan Powers2

  • 1Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.

The journal of physical chemistry. B
|August 19, 2025
PubMed
概括

可变温度原生电喷射电离-质谱学 (vT-nESI-MS) 揭示了缓冲区选择如何影响GroEL单环突变体 (SR1) 中的核酸结合. 温度和缓冲条件显著改变了蛋白质动力学和结合热力学,表明没有重大结构变化的全ostery.

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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a low-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.
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Mass Spectrometry: Isotope Effect

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Mass Spectrometry: Alkyne Fragmentation00:53

Mass Spectrometry: Alkyne Fragmentation

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