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斑马鱼中VX的毒性评估:使用DESI-MSI进行多器官毒性评估和组织分布可视化

Manzhu Cao¹, Yi Zhang¹, Mengxuan Dong¹

¹State Key Laboratory of Chemistry for NBC Hazards Protection, Beijing, 102205, China.

Archives of toxicology

|August 19, 2025

概括

化学战剂VX在斑马鱼中引起多器官毒性和生物积累. 雌性斑马鱼的VX积累明显高于雄性斑马鱼,突出了性别特异性风险.

科学领域:

毒理学毒理学毒理学
环境科学环境科学
化学武器制剂化学武器制剂化学武器制剂

背景情况:

作为化学战剂,VX构成了持续的威胁,需要对其毒性和生物积累进行研究.
了解VX的影响对于军事和反恐准备至关重要.
斑马鱼作为有毒学研究的有价值的模型生物,因为它们的透明度和快速发展.

研究的目的:

为了评估VX在斑马鱼中的多器官毒性.
为了确定VX的生物积累性质.
为绘制VX分布建立一个高分辨率的分析方法.

主要方法:

斑马鱼幼虫暴露于VX以确定急性毒性 (LC50和MNLC).
多器官毒性在低于致命度时被评估,通过组织学检查.
溶解电喷射电离-质谱成像 (DESI-MSI) 用于对VX及其代谢物进行空间映射.

主要成果:

确定的急性暴露LC50在409.98μg/L和MNLC在77.43μg/L.
暴露于VX导致斑马鱼幼虫的微,,退化和运动障碍.
组织学证实了VX诱导的多个器官损伤,包括大脑,脊髓和肝脏.
DESI-MSI揭示了VX和EMPA在九个器官中的生物分布.
生物积累因子分析显示,与雄性相比,雌性斑马鱼的VX积累是雌性斑马鱼的7.2倍.

结论:

在斑马鱼中,VX具有显著的多器官毒性和生物积累潜力.
这项研究强调了VX生物积累中明显的性别差异.
一个DESI-MSI框架提供了一个强大的工具,用于可视化化学战剂的分布,并协助风险评估.

关键词:

这就是 DESI-MSI.组织分布组织分布有毒性有毒性这是VX VX VX.斑马鱼是一种斑马鱼.

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