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转录基因景观和染色质可访问性揭示了关键调节器,驱动了化丝虫中被编程的幼虫-幼虫变

Yun Wang¹, Xin Yang¹, Junfeng Hong²

¹Laboratory of Evolutionary and Functional Genomics, School of Life Sciences, Chongqing University, Chongqing, China.

|August 19, 2025

概括

昆虫的变是由20-基乙 (20E) 调节的. 这项研究揭示了变过程中的动态基因表达和染色质变化,确定了皮层形成的新调节者,如C/EBP和βFTZ-F1.

科学领域:

发展生物学发展生物学
分子内分泌学分子内分泌学
昆虫生理学昆虫生理学

背景情况:

昆虫化,或生化,是一种关键的发育过程,涉及外骨脱落和形成新的皮质.
类固醇激素20-hydroxyecdysone (20E) 是昆虫化的关键调节剂,可以动态控制基因表达.
对于在变过程中随着20E水平的波动而发生的转录和表观基因组变化,人们的理解有限.

研究的目的:

为了研究表皮转录组的时间动态和丝虫 (Bombyx mori) 幼虫-幼虫变周期期间全基因组的色素可访问性.
为了确定参与表皮重塑和皮质形成的关键调节因素,由20E脉冲编排.

主要方法:

对表皮转录组的时间动态和Bombyx mori在变过程中的全基因组染色质可访问性的分析.
染色体足迹分析以确定转录因子结合部位.
对C/EBP和βFTZ-F1.1进行表皮特异性基因淘汰实验.

主要成果:

在早期和晚期变阶段之间观察到基因表达和染色质结构的明显变化,与20E标位变化相关.
埃克迪松受体 (EcR) 和粒状头 (GRH) 被确定为早期调节剂.
在晚期变阶段发现了一种涉及CCAAT/增强剂结合蛋白 (C/EBP) 和Fushi-tarazu f1 (βFTZ-F1) 的新型调节轴,C/EBP在20E下降时调节βFTZ-F1的表达. 这些基因的淘汰会损害皮质的形成.

结论:

这项研究揭示了协调昆虫变过程中的表皮重塑的动态调节电路.
涉及C/EBP和βFTZ-F1的等级转录级联控制着由20E波动驱动的皮质体更新.
这些发现增强了对激素介导的发育过渡和昆虫皮质形成的理解.

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