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从刺激周期中拯救IVM:改善卵细胞形态和能力

C Hilali¹, N Louanjli², M Ennaji³

¹Oncopathology Cancer Biology and Environment Laboratory, Mohammed VI Faculty of Medicine, Mohammed VI University of Health and Sciences (UM6SS), 21000 Casablanca, Morocco; Labomac Laboratory of Medical Analyses, Reproductive Biology, Casablanca, Morocco; IVF Laboratory, IRIFIV Fertility Center, Casablanca, Morocco.

Morphologie : bulletin de l'Association des anatomistes

|August 19, 2025

概括

一种新的体外成熟 (IVM) 培养基在辅助生殖技术中显著增强了卵细胞成熟和胚胎发育. 这种改进的介质提高了受精率,并创造了高质量的胚胎,以改善妊娠结果.

科学领域:

生殖生物学和医学生殖生物学和医学
辅助生殖技术 (ART) 是一种助孕技术.
卵细胞生物学和成熟过程

背景情况:

卵细胞体外成熟 (IVM) 对于辅助生殖至关重要,特别是用于拯救不成熟的卵细胞.
现有的IVM协议在实现最佳成熟和发展潜力方面面临挑战.
需要改进IVM培养基,以提高卵细胞质量和随后的胚胎发育.

研究的目的:

为了评估一种新的,内部配制的IVM培养基的疗效.
评估新介质对卵细胞成熟,发育能力和胚胎质量的影响.
将新媒体的性能与商业IVM媒体进行比较.

主要方法:

一项前性研究涉及60名接受细胞内质精子注射 (ICSI) 的患者.
未成熟的卵细胞 (胚芽囊和第一个阶段) 被分为两组:标准的商业IVM介质 (M组) 和商业介质,补充了专有细胞因子,介质蛋白和生长因子 (MS组).
评估了卵细胞成熟率,ICSI后受精率,以及第3天8细胞胚胎的比例. 此外,还比较了爆破率.

主要成果:

与M组相比,MS组表现出明显更高的卵细胞成熟率.
在MS组中,受精率和第3天8细胞胚胎的百分比显著增加.
与M组相比,MS组的爆发率显著改善 (P=0.02).

结论:

开发的IVM介质配方有效地改善了来自刺激周期的未成熟卵细胞的救援成熟.
这种增强的介质可以增加优质胚胎的产量,从而有可能提高怀孕率.
这种新的IVM介质通过增强胚胎发育和质量来优化ART结果的承诺.

关键词:

胚胎发育的发展在受精过程中受精.在体外进行IVM 媒体补充IVM媒体补充.卵子细胞在体外成熟救援救援

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