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细胞破坏技术对Cordyceps militaris蛋白质分离物的结构,功能和消化能力的影响
Houle Xiang1, Yuqing Zhang1, Shuo Zhang1
1College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
International journal of biological macromolecules
|August 19, 2025
概括
高压同质化 (HPH) 和超声波 (US) 显著增强真菌蛋白质提取并改善技术功能性质. 这些方法为在食品应用中利用真菌蛋白提供了可持续的解决方案.
科学领域:
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
- 生物化学 生化学
背景情况:
- 从真菌生物质中有效提取细胞内蛋白质对于食品应用至关重要.
- 了解细胞破坏方法对蛋白质结构和功能的影响是有限的.
研究的目的:
- 评估高压均质化 (HPH),超声波 (US) 和细胞酶水解用于提取Cordyceps militaris蛋白质分离物 (CMPI).
- 评估这些破坏技术对CMPI的结构和技术功能特性的影响.
主要方法:
- 三种细胞破坏技术 (HPH,US,细胞酶水解) 与仅取相比较.
- 分析了CMPI的结构性质 (二次结构,疏水性,二硫化键) 和技术功能性质 (水/油保持,发泡,乳化,可消化性).
主要成果:
- 所有的干扰方法都改善了蛋白质提取,HPH显示出最高的产量 (70.75%).
- 干扰改变了蛋白质结构 (减少α螺旋/β片,增加β转/随机线圈) 和增加表面疏水性.
- 通过HPH和US处理,获得了更高的水持量 (高达6.23g/g),油持量 (高达9.97g/g),泡 (高达69.57%) 和乳化能力 (高达32.07m2/g).
- 在体外消化能力显著提高了美国 (80.05%) 和HPH (71.71%).
结论:
- 细胞破坏技术,特别是HPH,显著提高了真菌蛋白质提取效率.
- 这些方法提高了真菌蛋白质分离物的结构灵活性和技术功能性质.
- 优化细胞破坏为食品工业中可持续利用真菌蛋白提供了潜在的潜力.
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