通过病理证据证实了腺病毒感染和肠接之间的关系
Lung-Huang Lin1, Chi-Jung Huang2, Cheng-Yu Lo3
1Department of Pediatrics, Cathay General Hospital, Taipei City, Taiwan.
Journal of clinical pathology
|August 19, 2025
概括
腺病毒感染与婴儿内受孕直接相关. 肠道组织的聚合酶连锁反应 (PCR) 测试比免疫组织化学 (IHC) 更敏感,以确认在肠接病例中这种腺病毒联系.
科学领域:
- 儿科胃肠病学 儿科胃肠病学
- 病毒病原体的产生
- 手术病理学手术病理学
背景情况:
- 婴儿内的病因往往是未知的,不同于老年儿童,有时会发现淋巴瘤或息肉等病理原因.
- 以前的研究表明,腺病毒感染和儿科内肠接之间存在联系,但证据主要依赖于间接来源,如便或喉拭子.
- 肠道组织的直接分析提供了一种更明确的方法来调查腺病毒-肠接关系.
研究的目的:
- 在6岁以下的儿童中直接调查腺病毒感染和肠接之间的关联.
- 用免疫组织化学 (IHC) 和聚合酶连锁反应 (PCR) 来分析肠道组织样本以检测腺病毒的证据.
- 为了比较IHC和PCR的诊断实用性和灵敏性,用于检测肠道接中的腺病毒.
主要方法:
- 儿科患者 (年龄小于6岁) 进行了回顾性评估,这些儿科患者患有需要手术的内肠接收.
- 将外科病理组织加工成甲固定式嵌 (FFPE) 片段进行分析.
- 在组织样本上应用腺病毒免疫组织化学 (IHC) 和PCR测试,与接受偶发尾切除术的儿童的对照组进行.
主要成果:
- 通过IHC检测出腺病毒在37.5%的肠接组织样本中,通过PCR检测出50%的腺病毒 (特别是腺病毒型C).
- 所有对照组的样本通过IHC和PCR检测呈阳性.
- 通过PCR检测,在被研究的内接组织中检测腺病毒的灵敏度高于IHC.
结论:
- 这项研究提供了直接的病理证据,证实了幼儿腺病毒感染和肠接之间的联系.
- 聚合酶连锁反应 (PCR) 是一种比免疫组织化学 (IHC) 更敏感和可靠的方法,用于诊断儿科内肠接病例中的腺病毒.
相关概念视频
01:22Appendicitis-I: Introduction
620
The appendix, a small, narrow, blind tube extending from the inferior part of the cecum, is widely regarded as a vestigial organ, having lost much of its original function through evolution. Despite its diminished role, the appendix can become inflamed, a condition known as appendicitis.
Etiology: Appendicitis can arise from various causes, primarily rooted in the obstruction of the appendix lumen. Factors contributing to this obstruction include fecal accumulation, lymphoid hyperplasia and, in...
Etiology: Appendicitis can arise from various causes, primarily rooted in the obstruction of the appendix lumen. Factors contributing to this obstruction include fecal accumulation, lymphoid hyperplasia and, in...
620
01:29Appendicitis-II: Diagnostic Studies and Management
145
Diagnosing and managing appendicitis requires a structured and comprehensive approach that spans from initial assessment to postoperative care. Here is an overview of the process:
Diagnosing Appendicitis
It requires a multifaceted approach, starting with a detailed physical examination to pinpoint the location and nature of the pain and identify any associated symptoms. Laboratory tests play a crucial role. A complete Blood Count (CBC) typically reveals leukocytosis (an increased number of...
Diagnosing Appendicitis
It requires a multifaceted approach, starting with a detailed physical examination to pinpoint the location and nature of the pain and identify any associated symptoms. Laboratory tests play a crucial role. A complete Blood Count (CBC) typically reveals leukocytosis (an increased number of...
145
01:26Histology of the Large Intestine
1.6K
The large intestine, a vital component of the gastrointestinal tract, is structured with four main layers: the mucosa, submucosa, muscularis, and serosa. Each layer performs a distinct role in facilitating the smooth functioning of the large intestine.
The innermost mucosa layer comprises simple columnar epithelium, lamina propria, and muscularis mucosae. This layer is primarily populated with absorptive cells, tasked with water absorption, and goblet cells, responsible for secreting mucus to...
The innermost mucosa layer comprises simple columnar epithelium, lamina propria, and muscularis mucosae. This layer is primarily populated with absorptive cells, tasked with water absorption, and goblet cells, responsible for secreting mucus to...
1.6K
01:23Anatomy of the Intestines
73.9K
Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
73.9K