菌株Streptomyces spp.的抗微生物活性. 从豆根球土壤中分离出来,对抗Xanthomonas sp
Jesús Rafael Trinidad-Cruz1, Gabriel Rincón-Enríquez1, Zahaed Evangelista-Martínez2
1Laboratorio de Fitopatología, Biotecnología Vegetal, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., El Bajío del Arenal, C.P. 45019, Zapopan, Jalisco, Mexico.
Revista Argentina de microbiologia
|August 19, 2025
概括
这项研究将Streptomyces细菌从土壤中分离出来,使用热或微波处理. 几种分离物对Xanthomonas sp显著的抗微生物活性,为农业疾病管理提供了潜在的新来源.
科学领域:
- 微生物学 微生物学
- 农业科学 农业科学
- 生物技术是生物技术.
背景情况:
- 菌株属Streptomyces的物种因其在农作物中的细菌疾病管理方面的潜力而得到认可.
- 树球土壤是各种微生物的丰富来源,具有潜在的生物控制特性.
研究的目的:
- 通过物理预处理从根球土壤中分离和识别类似于Streptomyces的动态细菌.
- 评估这些分离物对Xanthomonas sp.的抗菌活性.
主要方法:
- 来自豆种植园的树球土壤被 subjected 干热或微波辐射用于隔离actinobacteria.
- 使用双亚格层方法对Xanthomonas sp.进行了评估,以评估其抗菌活性.
- 使用16S rRNA基因序列分析进行了分离物鉴定.
主要成果:
- 分别使用干热和微波预处理获得了73和23种类似于Streptomyces的分离物.
- 34个干热隔离物 (68%) 和15个微波隔离物 (65%) 显示对Xanthomonas sp.有抑制作用.
- 三个高度活跃的分离物被确定为Streptomyces sp. BVBZ 35,BVBZ 47,和BVBZMW 18,与已知的Streptomyces物种具有很高的序列一致性.
结论:
- 物理预处理,特别是微波辐射,是有效的,以隔离强大的抗菌物种Streptomyces从土壤中.
- 已识别的Streptomyces分离物代表了开发农业中抗击Xanthomonas疾病的新生物控制剂的有希望的来源.
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