通过Ganoderma lucidum的基因甲基化,PRMT5通过调节细胞酶基因表达来促进细胞酶的产生
Yefan Li1, Zi Wang1, Zhouyu Li1
1Key Laboratory of Agricultural Environmental Microbiology, Microbiology Department, College of Life Sciences, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, People's Republic of China.
Microbial cell factories
|August 20, 2025
概括
蛋白质氨酸甲基转移酶5 (PRMT5) 在Ganoderma lucidum中促进细胞酶的产生. PRMT5通过基因组甲基化调节细胞酶基因表达,影响工业和环境应用.
科学领域:
- 生物化学
- 分子生物学
- 菌群学
背景情况:
- 细胞酶对纤维素降解至关重要,影响自然碳循环,工业过程和生物能源.
- 白色腐烂真菌Ganoderma lucidum是一个重要的细胞酶生产者.
- 已知翻译后的修改会影响细胞酶的产生.
研究的目的:
- 调查蛋白质氨酸甲基转移酶5 (PRMT5) 在Ganoderma lucidum中调节细胞酶的作用.
- 阐明PRMT5影响细胞酶合成的机制.
主要方法:
- 在Ganoderma lucidum中抑制prmt5的基因.
- 测量细胞酶活性和菌根生长速度.
- 农业废弃物降解的分析.
- 染色体免疫沉,然后进行定量PCR (ChIP-qPCR) 来评估PRMT5结合和基因组甲基化.
- 2 (eg2) 静止和过度表达菌株的构建和分析.
主要成果:
- 抑制prmt5显著降低了23%的细胞酶活性和71%的菌生长.
- PRMT5降低了大约60%和51%的玉米和玉米的降解.
- 通过基因组甲基化,ChIP- qPCR表明PRMT5通过基因组甲基化积极调节内葡萄糖酶2 (eg2) 表达.
- 过度表达EG2增强了细胞酶活性,证实了其在该途径中的作用.
结论:
- 在Ganoderma lucidum中,PRMT5对细胞酶的产生起着积极的调节作用.
- 该机制涉及PRMT5调节细胞酶基因表达的基因甲基化.
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