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相关概念视频

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

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Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
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Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

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The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
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Histone Modification02:32

Histone Modification

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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
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Master Transcription Regulators02:23

Master Transcription Regulators

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

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Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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相关实验视频

Updated: Sep 10, 2025

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
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通过Ganoderma lucidum的基因甲基化,PRMT5通过调节细胞酶基因表达来促进细胞酶的产生

Yefan Li1, Zi Wang1, Zhouyu Li1

  • 1Key Laboratory of Agricultural Environmental Microbiology, Microbiology Department, College of Life Sciences, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, People's Republic of China.

Microbial cell factories
|August 20, 2025
PubMed
概括

蛋白质氨酸甲基转移酶5 (PRMT5) 在Ganoderma lucidum中促进细胞酶的产生. PRMT5通过基因组甲基化调节细胞酶基因表达,影响工业和环境应用.

关键词:
细胞质其他甲基化其他:

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科学领域:

  • 生物化学
  • 分子生物学
  • 菌群学

背景情况:

  • 细胞酶对纤维素降解至关重要,影响自然碳循环,工业过程和生物能源.
  • 白色腐烂真菌Ganoderma lucidum是一个重要的细胞酶生产者.
  • 已知翻译后的修改会影响细胞酶的产生.

研究的目的:

  • 调查蛋白质氨酸甲基转移酶5 (PRMT5) 在Ganoderma lucidum中调节细胞酶的作用.
  • 阐明PRMT5影响细胞酶合成的机制.

主要方法:

  • 在Ganoderma lucidum中抑制prmt5的基因.
  • 测量细胞酶活性和菌根生长速度.
  • 农业废弃物降解的分析.
  • 染色体免疫沉,然后进行定量PCR (ChIP-qPCR) 来评估PRMT5结合和基因组甲基化.
  • 2 (eg2) 静止和过度表达菌株的构建和分析.

主要成果:

  • 抑制prmt5显著降低了23%的细胞酶活性和71%的菌生长.
  • PRMT5降低了大约60%和51%的玉米和玉米的降解.
  • 通过基因组甲基化,ChIP- qPCR表明PRMT5通过基因组甲基化积极调节内葡萄糖酶2 (eg2) 表达.
  • 过度表达EG2增强了细胞酶活性,证实了其在该途径中的作用.

结论:

  • 在Ganoderma lucidum中,PRMT5对细胞酶的产生起着积极的调节作用.
  • 该机制涉及PRMT5调节细胞酶基因表达的基因甲基化.