在NET-enanthate (NET-EN) 注射避孕药的使用者中,血清诺乙 (NET) 水平大大干扰丸激素免疫测试测量,并混生物学结果的解释
Chanel Avenant1, Johnson Mosoko Moliki2, Alexis J Bick2
1Department of Molecular and Cell Biology, University of Cape Town, Upper Campus, Private Bag, Rondebosch, Cape Town, Western Cape, 7700, South Africa. chanel.avenant@uct.ac.za.
Contraception and reproductive medicine
|August 20, 2025
概括
诺瑞乙 (NET-EN) 注射避孕药使用化学发光微粒免疫测试 (CMIA) 干扰丸激素测量. 这种干扰显著混了结果,显示NET-EN使用者的水平错误地升高.
科学领域:
- 内分泌学
- 生殖健康
- 临床化学
背景情况:
- 诺瑞 (NET) 和其乙酸盐形式 (NET-EN) 是女性广泛使用的注射避孕药.
- 之前的研究表明,口服NET可以通过化学发光微粒免疫测试 (CMIA) 干扰丸激素测量.
- 从使用NET-EN对CMIA的血清NET的影响尚未研究.
研究的目的:
- 研究使用NET-EN注射避孕药的女性血清NET是否会干扰测量.
- 在NET-EN和DMPA-IM使用者中,通过CMIA和超高性能液体染色体协同质谱法 (UHPLC-MS/MS) 测量的度进行比较.
主要方法:
- 在临床试验中采集了女性的血清样本,使用的是NET-EN或储存的美德乙酸肌肉避孕药 (DMPA- IM).
- 使用Abbott Architect CMIA和UHPLC- MS/ MS测量了的度.
- 从基线 (D0) 和启动后25周 (25W) 分析了匹配的样本.
主要成果:
- 与UHPLC-MS/MS相比,在NET-EN组中,CMIA显示度高出271%.
- 在NET-EN手臂中,CMIA表明丸激素从D0增加到25W,这与UHPLC-MS/MS发现的下降相矛盾.
- 在DMPA- IM手臂中,两种方法都显示的显著下降从D0到25W,两种方法之间没有显著差异.
结论:
- 在绝经前的NET-EN使用者中,生理NET度显著干扰着CMIA的度测定.
- 这种干扰更明显,并且发生在较低的NET度,而不是先前报告的口服NET度.
- 观察到的干扰使得对NET-EN对水平的影响的评估和与DMPA-IM的比较困难.
相关概念视频
Birth Control Methods
2.4K
Vasectomy is a surgical form of male sterilization that involves severing and sealing the vasa deferentia, preventing sperm from mixing with semen during ejaculation. Because a vasectomy does not impact the testes' ability to produce testosterone, hormone levels, libido, and sexual function generally remain unchanged. While vasectomy is highly effective in preventing pregnancy, with a success rate near 99.85%, rare cases of recanalization (spontaneous reconnection) can occur. Although...
2.4K
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
620
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
620
Drug Concentrations: Measurements
582
Drug concentration is the quantity of a drug present in a biological sample. Measuring drug amounts in biological samples allows the clinician to understand how a drug is absorbed, distributed, metabolized, and excreted. Samples can be obtained through invasive or non-invasive methods. Invasive techniques involve surgical or parenteral interventions to gather blood, cerebrospinal fluid, or tissue biopsy. Conversely, non-invasive approaches provide samples like urine, feces, and saliva.
Plasma...
Plasma...
582
Atomic Absorption Spectroscopy: Interference
1.0K
Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
1.0K
Target Cell Response to Hormones
3.5K
Hormones intricately bind to receptors on the surface or within target cells, initiating a cascade of cellular responses.
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
3.5K


