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相关概念视频

Autophagy01:27

Autophagy

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Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
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Aging01:26

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Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
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The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
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Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
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Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
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Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
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Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
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相关实验视频

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Ferritinophagy: Assessing the Selective Degradation of Iron by Autophagy in Human Fibroblasts
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通过中和自抑制组织激素DBI/ACBP来缓解病态衰老

Léa Montégut1,2, Flavia Lambertucci1,2, Lucas Moledo-Nodar3

  • 1Team "Metabolism, Cancer & Immunity", Centre de Recherche des Cordeliers, Equipe Labellisée par la Ligue Contre le Cancer, Université Paris Cité, Sorbonne Université, Inserm U1138, Institut Universitaire de France, Paris, France.

Autophagy
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概括

胰岛素结合抑制剂 (DBI/ ACBP) 是一种随着年龄和疾病而增加的激素. 中和DBI/ACBP通过减少衰老病态和促进健康显示治疗潜力.

关键词:
化学疗法发生DNA损伤年龄科学心脏衰竭这样就好了

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科学领域:

  • 生物化学
  • 老年学
  • 分子生物学

背景情况:

  • 甲结合抑制剂/ 甲结合蛋白 (DBI/ ACBP) 是一种抑制自的组织激素.
  • 随着年龄和疾病严重程度的增加,DBI/ ACBP的血水平与不良的临床结果相关.
  • DBI/ACBP升高与并发症,炎症和功能下降有关.

研究的目的:

  • 研究DBI/ACBP在衰老和与衰老相关的疾病中的作用.
  • 在临床前模型中评估中和DBI/ACBP的治疗潜力.

主要方法:

  • 在百岁以上的患者中分析DBI/ACBP血水平和与临床参数的相关性.
  • 在老化和疾病的小鼠模型中使用抗DBI/ACBP单克隆抗体.
  • 通过单核RNA测序评估各种生理和分子参数,包括功能,细胞衰老标记 (CDKN1A/p21) 和基因表达.

主要成果:

  • 百岁以上的患者,尤其是住院患者的DBI/ACBP水平显著更高,与不良临床因素相关.
  • 抗DBI/ ACBP治疗改善了与衰老相关的多种病理,包括生理功能和器官健康.
  • 在衰老模型中,DBI/ ACBP 中和抑制了纤维化和细胞衰老.
  • 在心脏和肝脏衰老模型中,抗DBI/ ACBP降低了衰老标志物并恢复了保护性基因表达模式.

结论:

  • DBI/ACBP既是病态衰老的标志物,也是病态衰老的驱动因素.
  • DBI/ACBP的中和表明多器官抗衰老作用.
  • 针对DBI/ACBP是一个有前途的治疗策略,