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一个E. 大肠杆菌双杂交系统用于研究人类蛋白质与蛋白质的相互作用

Rebecca M Richardson1, Sarah M Ho1, Lane Tong2

  • 1Department of Chemistry and Biochemistry, Old Dominion University, Norfolk, VA, USA.

BioTechniques
|August 20, 2025
PubMed
概括

在LexA-E. coli双杂交系统中验证了哺乳动物蛋白的相互作用,特别是GRP78与cl-Par-4的结合. 这种方法在没有真核细胞的情况下研究人类蛋白相互作用是有效的.

关键词:
细菌双杂交大肠杆菌β-银酸酶检测莱克萨麦克康尼亚格试剂葡萄糖调节蛋白质 78前列腺亡反应-4蛋白与蛋白的相互作用双混合动力

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