在 Porphyromonas gingivalis 中由氨酸酸酶控制的转录调节
Gatphan Atassi1, Kendall S Stocke1, Richard J Lamont1
1Department of Oral Immunology and Infectious Diseases, School of Dentistry, University of Louisville, Louisville, Kentucky, USA.
Molecular oral microbiology
|August 20, 2025
概括
在 Porphyromonas gingivalis 中,氨酸酸酶 Ltp1 和 Php1 影响基因表达,特别是在体内. Ltp1控制了对宿主条件的适应,而这两种酸酶则调节了输送系统.
科学领域:
- 微生物学
- 细菌转化后的变化
- 转录组学
背景情况:
- 氨酸酸化/脱酸化 (PTM) 调节细菌蛋白的功能和位置.
- 调节蛋白的PTM会影响细菌的基因表达.
- 氨酸酸酶对细菌转录组的总体影响在很大程度上是未知的.
研究的目的:
- 研究主要氨酸酸酶Ltp1和Php1对Porphyromonas gingivalis转录物的全球影响.
- 为了比较野生型和突变菌株的体外和体内转录特征.
- 阐明Ltp1和Php1在细菌适应和基因调节中的作用.
主要方法:
- 在 Porphyromonas gingivalis 野生型 (WT), Δltp1 和 Δphp1 突变菌株上进行了 RNA 测序 (RNA-Seq).
- 菌株在体外和体外 (老鼠) 条件下进行培养和测试.
- 进行了差异基因表达分析以确定DEG.
主要成果:
- 在体外,与WT相比,Δltp1和Δphp1突变体的转录差异很小.
- 在体内,Δphp1突变体表现出与传送系统相关的DEG.
- 在体内,Δltp1突变体显示DEG参与基因组稳定性和与Δphp1共享的传输系统调节.
- 在体内与体外相比,WT菌株显示DNA代谢过程 (重组,转移) 的调节.
结论:
- 在转录水平上,Ltp1在细菌适应体内环境方面发挥着重要作用.
- 在调节转运系统基因表达方面,Ltp1和Php1都有共同的功能.
- 氨酸酸酶是细菌基因表达的关键调节剂,特别是在宿主相互作用期间.
相关概念视频
Prokaryotic Transcriptional Activators and Repressors
21.8K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
21.8K
Global Regulatory Systems
71
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
71
Repressible Operon: trp Operon
135
The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
135
Operons
49.7K
Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...
49.7K
Gene Regulation in Microbial Communities: Quorum Sensing
92
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
92
Transcription Attenuation in Prokaryotes
16.0K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
16.0K


