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视觉化纳米粒子外流的次秒动态

Guoying Wang¹, Yueying Cao², Jia Li^1,3

¹Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, New South Wales 2109, Australia.

bioRxiv : the preprint server for biology

|August 20, 2025

概括

这项研究引入了实时光成像与上转化纳米粒子,以监测体内血管透性. 这项技术精确地追踪纳米粒子的移动, 揭示不同的传输途径, 改善疾病诊断和药物输送.

科学领域:

生物医学工程
纳米技术
血管生物学

背景情况:

改变血管完整性是各种疾病的关键指标.
监测血管透性对于了解疾病机制和开发新疗法至关重要.
目前用于评估血管透性的方法在敏感性和特异性方面存在局限性.

研究的目的:

开发一种新的实时光成像技术,用于在体内可视化和量化血管透性.
区分跨细胞和准细胞的运输途径.
评估血脑屏障 (BBB) 的完整性,并测试基于纳米粒子的BBB扰动策略.

主要方法:

使用基于化物的上转化纳米粒子作为光成像的对比剂.
在视频频率下同时采用血管和单颗粒的同焦成像.
在活生生的斑马鱼幼虫中进行高通量监测,以检测纳米粒子外流.

主要成果:

与传统光染料相比,在可视化血管透性方面获得了更高的灵敏度和特异性.
基于纳米粒子外流的次秒动力学区分跨细胞和半细胞运输.
使用聚酸盐功能化纳米颗粒的BBB透率增加了36.5倍,表明细胞间交叉增强.

结论:

开发的成像技术可以准确地实时监测血管完整性和内皮传输.
这种方法为推进血管生物学研究,疾病诊断和药物输送提供了强有力的工具.
功能化纳米颗粒对跨越生物障碍的药物提供有希望.

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