相关实验视频
Updated: Sep 8, 2025

12:15
In Vitro Polymerization of F-actin on Early Endosomes
Published on: August 28, 2017
9.2K
中体P4.1关联蛋白 (CPAP) 是多细胞体形成和内体成熟过程中ESCRT通路功能的新型调节剂
bioRxiv : the preprint server for biology
|August 20, 2025
概括
中体P4. 1关联蛋白 (CPAP) 将TSG101引入内体,促进内体成熟和内细胞囊运输途径. 这揭示了CPAP在ESCRT介导的内分体功能中的新角色.
科学领域:
- 细胞生物学
- 分子生物学
- 走私蛋白质
背景情况:
- 已知中心体P4. 1相关蛋白 (CPAP) 调节内细胞囊运输 (EVT) 和表皮生长因子受体 (EGFR) 的溶酶体向.
- 通过CPAP影响EVT和内分体成熟的确切机制尚未完全阐明.
研究的目的:
- 研究CPAP促进EVT和内体成熟的机制.
- 确定CPAP在ESCRT组件到内分体的招募中的作用.
主要方法:
- 共同免疫沉测试以评估蛋白质相互作用.
- 免疫光显微镜以确定蛋白质的同位化.
- 使用CPAP的细胞消耗研究.
- 对ESCRT组件的过度表达研究.
主要成果:
- CPAP与TSG101,一种ESCRT- I蛋白直接相互作用,并与TSG101和HRS (ESCRT- 0) 在早期内分体上同位.
- CPAP的耗尽会扰乱Rab5- Rab7转换和内分体成熟,但不是通过与这些GTPase的直接相互作用.
- 在CPAP枯竭的细胞中,HRS的过度表达恢复了TSG101的招募和内体成熟,表明CPAP在TSG101的上游和HRS的平行功能.
结论:
- CPAP通过招募TSG101到内分泌体,从而促进ESCRT的功能,起到内分泌体成熟的关键调节作用.
- CPAP跨越ESCRT-0和ESCRT-I复合体,揭示了这种中心体生物生成蛋白在内体贩运中的新型ESCRT-依赖的调节作用.
相关概念视频
Intralumenal Vesicles and Multivesicular Bodies
3.7K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.7K
ER Retrieval Pathway
3.9K
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
3.9K
The Early Endosome: Endocytosis of Transferrin
3.4K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
3.4K
Receptor Downregulation in MVBs
2.1K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.1K
Coat Assembly and GTPases
3.6K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
3.6K
Recycling Endosomes and Transcytosis
2.8K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
2.8K

