沈元益智囊缓解冠状动脉微血管功能障碍的机制:网络分析和实验证据
Jiaping Chen1,2, Huiwen Zhou1, Hongxu Liu1
1Department of Cardiology, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing, China.
Frontiers in pharmacology
|August 20, 2025
概括
通过促进血管生长和减少细胞死亡,改善冠状动脉微血管功能障碍 (CMD). 这项研究通过miR-302-3p/Hippo途径阐明了它的机制.
科学领域:
- 心血管研究
- 中国传统医学药理学
- 分子生物学
背景情况:
- 冠状动脉微血管功能障碍 (CMD) 影响心脏健康,对治疗机制的理解有限.
- 通过调气和消除血液静止,Shenyuan Yiqi Huoxue囊 (SYYQ) 显示出对CMD的临床前景.
- 在CMD中SYQ有效性的精确分子机制需要阐明.
研究的目的:
- 研究SYYQ在缓解冠状动脉微血管功能障碍 (CMD) 的潜在机制.
- 探索网络药理学,体内和体外实验在了解SYYQ的治疗效果中的作用.
- 确定SYYQ对CMD的关键分子途径和目标.
主要方法:
- 使用网络药理学预测SYYQ对CMD的潜在治疗点和途径.
- 在体内研究中使用了通过心声学,组织病理学,血清生物化学,TUNEL和TEM评估的CMD大鼠模型.
- 在体外研究中,在低氧条件下使用小鼠心脏微血管内皮细胞 (MCMEC),采用细胞传染,TEM,西式涂抹和qRT- PCR.
主要成果:
- 网络分析表明SYYQ会影响CMD中的细胞亡和血管生长途径.
- 在大鼠中,SYYQ治疗减少了细胞亡,增强了微血管通透性,并减轻了心肌损伤.
- 在实验室中,SYYQ抑制了细胞亡,促进了血管生成因子,降低了miR-302-3p的调节,并调节了缺氧性MCMEC中的Hippo通路.
结论:
- 通过促进血管生成因子的产生和抑制亡,SYYQ有效地缓解CMD.
- 该机制涉及miR-302a-3p/Hippo通路,其中SYQ调节miR-302a-3p水平和Hippo通路活性.
- 通过这种特定的分子轴,SYYQ的治疗效果与其调节血管生成和亡的能力有关.
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