相关实验视频
Updated: Sep 10, 2025
10:23
Glutamine Flux Imaging Using Genetically Encoded Sensors
Published on: July 31, 2014
9.7K
一个基因编码的生物传感器来描述谷氨酸的运输和代谢
Kaiyu Gao1, Hui Zhang1, Yidong Liu1
1State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, P. R. China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|August 20, 2025
概括
一个新的光生物传感器,Glusor,准确地量化生物样本和细菌中的谷氨酸. 这种工具有助于诊断谷蛋白酸性尿症和监测谷蛋白酸盐的产生,提供了多方面的 in vitro 和 in vivo 检测能力.
科学领域:
- 生物化学
- 代谢工程
- 分子生物学
背景情况:
- 谷氨酸是一种关键的平台化学物质和内源代谢物.
- 谷氨酸-CoA脱酶 (GcdH) 缺乏导致谷氨酸酸尿,这是一种遗传性代谢障碍.
- 精确的酸盐检测对于疾病诊断和代谢研究至关重要.
研究的目的:
- 开发和优化一种基因编码的光生物传感器,用于酸盐检测.
- 在各种生物和工业样本中使用生物传感器量化酸盐.
- 研究不同细胞系统中的酸盐运输,新陈代谢和积累.
主要方法:
- 使用CsiR和循环 permuted黄色光蛋白构建和优化Glusor生物传感器.
- 人体液体和细菌发酵中的酸盐量化.
- 在大肠杆菌中酸盐运输 (吸收和排放) 的特征.
- 在细菌,HEK293FT细胞和特定条件下 (GcdH抑制,低氧) 检测酸盐.
主要成果:
- 格卢索生物传感器在酸盐量化方面表现出高准确度和精度.
- 确定了KgtP和YnfM载体在大肠杆菌中酸盐吸收和排放中的作用.
- 在细菌和HEK293FT细胞中揭示了谷氨酸代谢途径,识别了谷氨酸基酶.
- 在体内分析酸盐积累的空间溶解,将其与GcdH抑制和缺氧联系起来.
结论:
- Glusor是一种多功能和可靠的 in vitro 和 in vivo 谷氨酸检测工具.
- 有助于方便地诊断谷氨酸尿和监测谷氨酸酸的产生.
- 能够更深入地了解各种生物系统中的酸盐运输和代谢.
相关概念视频
01:27Glucose Transporters
24.1K
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
24.1K
01:32Secondary Active Transport
7.6K
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
7.6K
01:30Membrane Proteins
21.2K
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
21.2K
01:44The Significance of Membrane Transport
29.5K
The transport of solutes across the cell membrane is essential for metabolic processes, like maintaining cell size and volume, generating the action potential, exchanging nutrients and gases, etc. Membrane transport can be either passive or active. It can be simple diffusion, facilitated, or mediated transport aided by transport proteins such as transporters and channels.
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
29.5K