相关实验视频

Updated: Sep 10, 2025

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Published on: March 9, 2018

基于新陈代谢的真菌球鼻炎的特征

Yi Wang^1,2, Hongzheng Wei^1,2, Ting Yang^1,2

¹Department of Otolaryngology-Head and Neck Surgery, Capital Medical University, Beijing, China.

Ear, nose, & throat journal

|August 20, 2025

概括

非目标代谢学显示在真菌球性鼻炎 (FBS) 中存在显著的代谢差异. 关键代谢物如氧化L- 氨酸和酸胆被改变,影响了真菌球中的核酸代谢.

科学领域:

耳鼻喉科
代谢学
菌群学

背景情况:

球性鼻炎 (FBS) 是一个需要手术的常见疾病.
了解真菌球的代谢环境对于阐明疾病机制至关重要.

研究的目的:

使用非目标代谢学来描述FBS的代谢变化.
确定不同代谢物及其在真菌感染和免疫反应中的潜在作用.

主要方法:

预期招募30名FBS患者和29名对照人群.
使用LC-MS/GC-MS进行真菌球和分泌物的代谢分析.
多变量统计分析 (PCA,OPLS-DA) 和KEGG通路丰富分析.

主要成果:

在阳性和负性电离模式下分别发现了7个和6个差异代谢物.
升调酸胆 (LPC O- 18: 0, LPC O- 20: 1) 和LPE O- 20: 1;降低氧化L- 氨酸和4- 乙胺酸的调节.
在真菌球中观察到核酸代谢途径的显著失调.

结论:

FBS真菌球中的代谢物组成和代谢途径与周围组织和对照细胞有显著差异.
这些代谢差异可能导致FBS的真菌感染和免疫失调.

关键词:

4 - 乙胺酸菌球性鼻炎化脂核酸代谢氧化的谷氨没有目标的代谢组

更多相关视频

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Published on: May 2, 2018

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

相关概念视频

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...