在易腐烂的蔬菜和水果中使用液态染色体双重质谱测量后的真菌杀菌剂的确认分析方法
Keiko Iwakoshi1, Hiroyuki Miyakawa1, Megumi Hasebe1
1Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Japan.
概括
一种新方法使用LC-MS/MS准确检测进口产品中的八种常见的收获后杀菌剂. 这种经过验证的技术通过提供可靠的定量限值来确保食品安全.
科学领域:
- 分析化学
- 食品科学
- 环境科学
背景情况:
- 收获后的杀菌剂对于保存进口水果和蔬菜至关重要.
- 准确可靠的检测方法对于食品安全和合规性至关重要.
- 现有的方法可能缺乏全面的真菌杀菌剂分析的灵敏度或范围.
研究的目的:
- 开发和验证八种主要的收获后杀菌剂的同时分析方法.
- 为进口产品建立一个敏感和可重复的量化技术.
- 通过有效的杀菌剂监测来支持常规的食品安全管理.
主要方法:
- 使用高性能液体色谱与并联质谱 (LC-MS/MS).
- 专门的固相提取 (SPE) 柱子用于样品净化.
- 酸提取和陶同质化用于样本制备.
主要成果:
- 该方法实现了所有八种杀菌剂的10μg/kg的量化极限.
- 恢复率在73. 2%至105. 3%之间,相对标准偏差较低 (0. 9%至24. 7%).
- 该方法符合日本的验证准则,显示出高可重复性和可靠性.
结论:
- 开发的LC-MS/MS方法为同时进行真菌杀菌剂分析提供了优越的方法.
- 这种技术适用于对各种水果和蔬菜的常规监测.
- 这种方法有助于确保进口产品的食品安全.
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