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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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相关实验视频

Updated: Sep 10, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

841

使用基于阿兹拉克的探针对G4DNA结构进行光照明

Annyesha Biswas1, Nitesh Ayare1, Y Dilnawaj1

  • 1Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.

Biochemistry
|August 20, 2025
PubMed
概括

新的光探针 (AZL1-3) 能够有效地检测G-四重复 (G4) DNA结构,包括c-MYC和c-KIT1中的结构. 这些探测器显示了生物成像和诊断的潜力.

科学领域:

  • 分子生物学
  • 生物化学
  • 化学生物学

背景情况:

  • 在DNA和RNA中富含G的序列形成G四重复 (G4) 结构.
  • G4结构在各种生物过程中起着至关重要的作用.
  • 了解G4拓,位置和功能在细胞和无细胞环境中至关重要.

研究的目的:

  • 开发用于G4结构检测的新型小分子光探针.
  • 对特定的G4拓进行探测基于亚兹拉克 (AZL1-3) 的探测能力.
  • 评估这些探针的细胞本地化和潜在应用.

主要方法:

  • 三个基于亚兹拉克的光探针的合成 (AZL1-3).
  • 光光谱测量探针结合和光照响应与G4DNAs (c-MYC,c-KIT1,HRCC).
  • 使用HeLa细胞进行细胞检测,以评估探针局部化和细胞毒性.

主要成果:

  • 对于c-MYC,c-KIT1和线粒体HRCCG4DNA的平行G4拓,AZL1-3探针显示出显著的光亮度 (65-135倍).
  • 领先的探测器AZL1显示了与c-KIT1 G4 DNA的2:1结合,与G四重奏相互作用.
  • 观察到有限的细胞毒性,探针显示在HeLa细胞中的细胞质局部化,包括与脂质滴滴的局部化.

更多相关视频

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

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相关实验视频

Last Updated: Sep 10, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

841
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

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结论:

  • 基于阿兹拉克的探针是有效的工具,用于在无细胞条件下检测G-四重体结构.
  • 开发的探测器显示出潜在的生物成像和诊断应用的前景.
  • 这些探测器的进一步工程可以提高它们在细胞G4检测中的实用性.