由Entamoeba histolytica引发的宿主细胞代谢重编程的多组学见解
Yanqing Zhao1, Hongze Zhang1, Shaokun Pan1,2
1Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, China.
Microbiology spectrum
|August 20, 2025
概括
中间子单元 (Igl) 的Entamoeba histolytica
科学领域:
- 细胞生物学
- 寄生虫学
- 代谢学
背景情况:
- 甲状腺菌 (Entamoeba histolytica) 是人类肠道的一种严重感染.
- Gal/GalNAc 乳素的中间亚单元 (Igl) 是E. histolytica的一个关键毒性因子.
- 了解Igl与宿主细胞的相互作用对于开发治疗至关重要.
研究的目的:
- 调查真核体表达Igl如何影响Caco-2细胞的代谢过程.
- 阐明Igl在诱导代谢重编程和宿主细胞自中的作用.
- 为了确定Entamoeba histolytica中Igl致病性的新机制.
主要方法:
- 针对性代谢分析以确定改变的代谢物.
- 代谢流量分析以研究代谢途径的活性.
- 分析宿主细胞反应的单细胞转录组学.
- 为了研究自途径,
主要成果:
- 在Caco-2细胞中检测到的232种代谢物中,Igl治疗显著改变了63种.
- Igl诱导了一种类似于沃尔堡的效应,增加了葡萄糖摄入量,乳酸生成和ATP生成,同时抑制了有氧呼吸.
- Igl激活了宿主细胞自,涉及了哺乳动物的拉巴胺途径.
- 单细胞转录组显示在特定宿主细胞组中抑制有氧呼吸和激活应激反应.
结论:
- E. histolytica的Igl诱导了新陈代谢重编程和宿主上皮细胞的瓦尔堡式转移.
- Igl激活有氧糖解并调节宿主细胞自,代表了一种新的毒性机制.
- 这些发现提供了关于氨菌病原和潜在治疗点的见解.
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