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在Lentinula edodes中受微RNA调节的温度影响的真菌病毒相互作用的新见解
Chun-Xi Liu1, Meng-Pei Guo1, Jun-Zhuo Zhou1
1Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, China.
Journal of virology
|August 20, 2025
概括
热应激增强了病毒的复制,并降低了宿主的耐热性. 一种真菌微RNA,led-milR-21,针对一个关键的耐热基因,在压力下影响病毒与宿主之间的相互作用.
科学领域:
- 菌群学
- 病毒学
- 植物病原体相互作用
- 分子生物学
背景情况:
- 中的病毒感染可能会导致大量的收获损失.
- 热应激是一种可以影响宿主-病原体相互作用的环境因素.
- 了解这些相互作用对于农业应用和管理真菌病至关重要.
研究的目的:
- 研究热应激 (HS) 对Lentinula edodes mycovirus HKB (LeV) 和其宿主Lentinula edodes之间的相互作用的影响.
- 阐明宿主对病毒感染和热应激反应的分子机制.
- 探索微RNA在这些相互作用中的作用.
主要方法:
- 转录组分析以评估HS和LeV感染下的基因表达变化.
- 参与热耐受性的宿主微RNA (led-milR-21) 的功能分析.
- 使用烟草短暂表达和qRT-PCR进行基因沉默测试以确认目标相互作用.
- 代谢途径分析以确定参与宿主反应的基因.
主要成果:
- 热应激 (HS) 显著增加了LeV复制,并降低了L. edodes的热耐受性.
- LeV感染降低了与热耐受性相关的基因, 并改变了宿主对HS的反应.
- 由LeV和HS诱导的宿主微RNAled-milR-21针对热耐受性转录因子LE01Gene01783.
- 参与氨基酸代谢,蛋白质加工和脂质代谢的基因与宿主反应有关.
结论:
- 通过诱导led-milR-21,LeV感染和HS协同损害L. edodes的热耐受性.
- 这项研究揭示了一种涉及真菌微RNA的新型病毒与宿主相互作用机制.
- 这些发现为结合病毒和非生物应激的真菌反应提供了洞察力,与气候变化情景相关.
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