探索复杂生物矩阵的直接和高通量选分析的金属有机框架涂层刀片
Isaac Negrín-Santamaría1,2, María J Trujillo-Rodríguez1,2, Encarnación Moyano3
1Laboratorio de Materiales para Análisis Químico (MAT4LL), Departamento de Química, Unidad Departamental de Química Analítica, Universidad de La Laguna (ULL), San Cristóbal de La Laguna 38108, Spain.
Analytical chemistry
|August 20, 2025
概括
这项研究引入了一种使用金属有机框架 (MOF) 在涂层叶片喷雾 (CBS) 中快速分析尿液中的化学物质的新方法. 这项技术能够在8分钟内检测出新生菌.
科学领域:
- 分析化学
- 材料科学
背景情况:
- 环境电离质谱 (AIMS) 提供简单,高通量分析.
- 涂层叶片喷雾剂 (CBS) 提高了生物流体分析的灵敏度.
- 金属有机框架 (MOF) 在捕获各种分析物方面表现出色.
研究的目的:
- 将清洁的MOF集成到CBS设备中,用于人类尿液中的异生物确定.
- 使用高分辨率质谱测量 (HRMS) 开发一种新的嫌疑人查工作流程.
- 扩大AIMS技术的化学覆盖范围.
主要方法:
- 通过简单的,温和的策略将整洁的MOF集成到CBS设备中.
- 开发与HRMS相结合的可疑选工作流程.
- 在人类尿样中分析异生菌.
主要成果:
- 通过使用MOF集成的CBS在人体尿液中成功确定异生菌.
- 达到的量化极限 (LOQ) 低至0.1 ng·mL-1.
- 整个过程的快速分析时间为8分钟.
结论:
- 清洁的MOF可以有效地集成到CBS设备中进行敏感的外来生物分析.
- 开发的HRMS嫌疑人选工作流程扩展了AIMS的能力.
- 这种方法为像人类尿液这样的复杂矩阵提供了快速,灵敏和高质量的分析方法.
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