Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Proteomics01:33

Proteomics

7.9K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Evaluating the Performance of Photon- and Electron-Based Fragmentation Methods in Omnitrap-LCMS Analysis of <i>N</i>-Glycopeptides.

Analytical chemistry·2026
Same author

Investigating the Efficiency of Ultraviolet Photodissociation in Peptides Modified with <i>N</i>-Terminal UV-Absorbing Chromophores.

Journal of the American Society for Mass Spectrometry·2026
Same author

Integration of alternative fragmentation techniques into standard LC-MS workflows using a single deep learning model enhances proteome coverage.

Nature methods·2026
Same author

Pre-assembly of biomolecular condensate seeds drives RSV replication.

Nature·2026
Same author

Biocompatible ligand balancing in transition metal coordination enables benign in-cell protein arylation.

Nature chemistry·2026
Same author

Unlocking Phytoplankton Metallomes with Comparative Analysis of Metal Quotas, Quantitative Proteomics, and Inferred Metalloproteomes.

Environmental science & technology·2025
Same journal

Modeling the Effects of Short-Range Randomness in Packed Sphere Beds.

Analytical chemistry·2026
Same journal

Mitochondrial Redox Cascade-Directed Covalent NIR Fluorogenic Imaging of Therapy-Induced Senescence Integrates Tumor and Host Responses.

Analytical chemistry·2026
Same journal

Proteomic Profiling of RHD-Related Mitral Annulus Calcification Enabled by Magnetic Carbon Nanomaterial-Supported Quasi-Immobilized Enzyme Digestion.

Analytical chemistry·2026
Same journal

Spatial-Photonic Encoding on a Single Fiber: Breaking the Bottleneck in Photoelectrochemical Biosensing for Precision Diagnostics.

Analytical chemistry·2026
Same journal

Spreadable Biosensing Pregel for Analyte Visualization in Peeled Plant Tissues.

Analytical chemistry·2026
Same journal

DARibo-Q: RNA Allosteric Transduction for Fluorescence Imaging of Dopamine Modulation in Living Systems.

Analytical chemistry·2026
查看所有相关文章

相关实验视频

Updated: Sep 10, 2025

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
11:16

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

Published on: July 12, 2014

37.2K

固体粒子是否已准备好进行超时蛋白质组?

Eduardo S Kitano1,2, Yana Demyanenko1,2, Shabaz Mohammed1,3,4

  • 1Rosalind Franklin Institute, Harwell Campus, OX11 0QX Didcot, United Kingdom.

Analytical chemistry
|August 20, 2025
PubMed
概括
此摘要是机器生成的。

非多孔的C-18粒子列为高速蛋白质学提供了高效的分离. 这些柱子的性能与纳米UHPLC和纳米HPLC系统中的传统材料相美.

更多相关视频

Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation
02:36

Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation

Published on: March 8, 2024

1.1K
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
11:12

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Published on: February 7, 2022

10.6K

相关实验视频

Last Updated: Sep 10, 2025

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
11:16

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

Published on: July 12, 2014

37.2K
Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation
02:36

Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation

Published on: March 8, 2024

1.1K
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
11:12

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Published on: February 7, 2022

10.6K

科学领域:

  • 分析化学
  • 生物化学
  • 染色学

背景情况:

  • 高通量蛋白质组学需要高效的分离技术.
  • 传统的染色学依赖于完全多孔的 (FPP) 和表面多孔的粒子 (SPP).
  • 无孔粒子 (NPP) 材料在速度和效率方面具有潜在的优势.

研究的目的:

  • 在高速蛋白质组学工作流程中评估无孔C-18静止相的性能.
  • 评估核电站柱对纳米UHPLC和纳米HPLC系统的适用性.
  • 将核电站柱的性能与FPP和SPP材料进行比较.

主要方法:

  • 使用1.0-1.5μm无孔C-18颗粒材料 (SOLAD和ODS-IIIE) 制造分析柱.
  • 使用150μm i.d的纳米UHPLC和纳米HPLC压力模式进行兼容性测试. 化的毛细血管.
  • 使用不同的列长 (15-25厘米) 和优化的HPLC方法 (Evosep One Whisper Zoom) 评估分离效率.
  • 通过DIA LC-MS/MS分析对人类细胞溶解物进行性能比较.

主要成果:

  • 在纳米UHPLC系统中,ODS-IIIE和SOLAD核电站材料都支持高效的分离.
  • 较短的核电站列实现了与FPP和SPP列相比的竞争性分离性能 (FWHM<2-3秒).
  • 根据DIA的LC-MS/MS分析,NPP柱的性能与FPP和SPP材料相比相当或略高.

结论:

  • 基于无孔粒子 (NPP) 的柱子是蛋白质组学中FPP和SPP材料的可行替代品.
  • 核电站柱特别适用于高通量和高灵敏性蛋白质组应用.
  • 这些发现支持在高级蛋白质组工作流程中采用核电站列.