相关实验视频

Updated: May 5, 2026

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

Published on: July 23, 2010

在HeLa细胞中核外的超结构可视化

Sourabh Sengupta¹, Zhaojie Zhang², Daniel L Levy³

¹Department of Molecular Biology, University of Wyoming, Laramie, WY, USA.

Methods in molecular biology (Clifton, N.J.)

|August 20, 2025

概括

传输电子显微镜 (TEM) 可视化细胞结构. 本研究详细介绍了一种用于观察HeLa细胞中的核外的TEM方法,帮助研究各种条件下的超结构.

科学领域:

细胞生物学
显微镜技术

背景情况:

自20世纪40年代以来,传输电子显微镜 (TEM) 已经可视化了生物材料.
TEM提供细胞器官,细胞内结构和病毒的详细观察.
使用TEM可以了解细胞核的结构和功能.

研究的目的:

用TEM呈现一种用于在培养的HeLa细胞中可视化核外的方法.
提供从细胞培养到可视化的综合方案.
为了研究由于细胞培养条件,遗传修饰和压力的核外超结构变化.

主要方法:

细胞培养HeLa细胞.
固定,嵌入,切割和染色协议.
使用传输电子显微镜进行可视化.

主要成果:

建立了一个详细的核包裹TEM可视化方法.
该协议允许核外超结构的高分辨率成像.
该方法适用于各种实验条件.

结论:

这种TEM方法对HeLa细胞的详细核外可视化是有效的.
这种技术有助于研究外部因素如何影响核外超结构.
这项研究为细胞生物学研究提供了宝贵的工具.

关键词:

甲基细胞核包裹中核传输电子显微镜

更多相关视频

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Published on: September 27, 2024

相关概念视频

Distribution of Cytoplasmic Content

Distribution of Cytoplasmic Content

Cytokinesis segregates a cell’s chromosomes and organelles into its daughter cells. Organelles divide and grow prior to cell division but cannot be synthesized de novo; therefore, cells must receive at least one copy of each organelle to survive. Currently, many of the details of how the organelles are distributed are not yet fully elucidated.
Distribution of cytoplasmic determinants
The cytoplasm contains various organelles, as well as salts, proteins, and water. The distribution of...