相关实验视频

Updated: May 6, 2026

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

配对Lamin B1-DamID和Immuno-3D-FISH以解决和验证脂肪生成的外围基因组组织

Rafal Czapiewski^1,2, Jose I de Las Heras³, Eric C Schirmer⁴

¹Institute of Cell Biology, University of Edinburgh, Edinburgh, UK. rafal.czapiewski@ed.ac.uk.

Methods in molecular biology (Clifton, N.J.)

|August 20, 2025

概括

这项研究优化了研究脂肪细胞空间基因组组织的DamID方法. 这些发现揭示了核膜蛋白如何影响基因定位,这对于理解特定组织的基因调节和疾病至关重要.

科学领域:

基因组学
细胞生物学
分子生物学

背景情况:

空间基因组组织是组织特异性的,对基因调节至关重要.
基因组组织的破坏与衰老,癌症和发育性疾病有关.
核膜蛋白与基因相互作用,但由于蛋白质不溶性,识别目标是具有挑战性的.

研究的目的:

为脂肪细胞中B1-DamID提供一个优化的方案.
在此背景下建立分析DamID数据的方法.
开发一个补充的3D免疫FISH技术来验证结果.

主要方法:

对前脂肪细胞和分化脂肪细胞进行了优化.
为DamID数据开发了两种不同的分析方法.
针对脂肪细胞建立了一个专门的3D免疫- FISH协议.

主要成果:

优化了B1-DamID协议,可以识别核外中的基因.
在核膜蛋白位控制下对基因进行比较分析.
3D免疫FISH证实了DamID在脂肪细胞中的结果,克服了自身光的挑战.

结论:

优化的DamID协议对于研究组织特异性基因组组织至关重要.
这项工作为研究脂肪细胞中的核膜蛋白-基因组相互作用提供了有价值的工具.
这些发现有助于理解空间基因组组织在健康和疾病中的作用.

关键词:

脂肪细胞脂肪生成一个DamID 鱼类美国拉米纳核包裹

更多相关视频

Robust 3D DNA FISH Using Directly Labeled Probes

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

Published on: January 27, 2016

相关概念视频

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.