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肝细胞癌中静脉瘤显著的免疫微环境

Kai-Qian Zhou^1,2,3, Yu-Chen Zhong^1,2, Min-Fang Song^4,5,6

¹Department of Liver Surgery & Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, China.

Hepatology (Baltimore, Md.)

|August 20, 2025

概括

门静脉瘤 (PVTT) 与肝细胞癌预后不佳有关. 与瘤相关的巨细胞 (TAMs),特别是C5aR+ TAMs,在PVTT中产生免疫抑制环境,从而恶化患者的结果.

科学领域:

肝细胞癌研究
瘤微环境免疫学
癌症生物标志物

背景情况:

门静脉瘤 (PVTT) 是肝细胞癌 (HCC) 的一个显著的负面预后因素.
PVTT与肝脏内扩散和门门高血压的增加有关.
在PVTT的特定免疫特征仍然在很大程度上未被探索.

研究的目的:

研究PVTT的免疫微环境.
确定关键的免疫细胞群及其在PVTT中的作用.
了解免疫微环境对PVTT预后的贡献.

主要方法:

用于免疫细胞分析的飞行时间质量细胞测量.
单细胞RNA测序 (scRNA-seq) 用于详细的细胞分析.
多重光免疫组织化学用于空间特征.

主要成果:

在PVTT中,巨细胞和单细胞是主要的免疫细胞,明显高于原发性瘤或血液.
通过丰富的抑制和调节性免疫细胞表现出免疫抑制的环境.
与瘤相关的表达C5aR的巨细胞 (C5aR+ TAMs) 是一个主要的亚群,与C5a水平相关,并抑制CD8+ T细胞活性.
C5aR+ TAM透与HCC差异化有关,是整体存活率和PVTT切除后复发的一个风险因素.

结论:

与瘤相关的巨细胞 (TAMs),特别是C5aR+ TAMs,在PVTT中显著丰富.
通过促进免疫抑制瘤微环境,C5aR+ TAM在促进PVTT发育和不良预后方面发挥着至关重要的作用.

关键词:

肝细胞癌免疫微环境门静脉瘤 / 瘤一个细胞的奥米克

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The Tumor Microenvironment

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