基于里埃的多切片重建在冷电子断层扫描

相关实验视频

Updated: Sep 10, 2025

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

5.2K

Ranhao Zhang1, Yuan Shen2, Xueming Li3

  • 1Key Laboratory for Protein Sciences of Ministry of Education, School of Life Sciences, Tsinghua University, Beijing 100084, China; Beijing Frontier Research Center for Biological Structure, Beijing 100084, China; Tsinghua-Peking Joint Center for Life Sciences, Beijing 100084, China; State Key Laboratory of Membrane Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; School of Life Sciences, Tsinghua University, Beijing 100084, China; Department of Electronic Engineering, Tsinghua University, Beijing 100084, China; Beijing National Research Center for Information Science and Technology, Tsinghua University, Beijing 100084, China.

Ultramicroscopy
|August 20, 2025
PubMed
概括

我们开发了MULtiple Slice技术 (MUST),一种用于冷电子断层扫描 (cryoET) 的新福里埃空间算法. 必须能够高效,高分辨率的断层图像重建和处理,克服当前方法的局限性.

关键词:
3D-CTF的纠正别名使用低温电子断层扫描在现场结构断层图像重建

更多相关视频

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

14.2K
Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

4.6K

相关概念视频

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.5K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.6K