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使用UPLC/Q-TOF-MS与LC-MS/MS验证进行鱼的特定物种识别

Jinju Zhang¹, Lidan Zhang², Jungang Lu³

¹School of Pharmacy, Jinan University, Guangzhou 510632, China; Key Laboratory of Traditional Chinese Medicine Quality Control and Safety Evaluation of Guangdong Provincial Drug Administration, Guangdong Institute for Drug Control, Guangzhou 510663, China.

Journal of pharmaceutical and biomedical analysis

|August 20, 2025

概括

这项研究引入了一种快速的UPLC/Q-TOF-MS和PCA方法来识别松鼠 (PS) 种类. 开发的LC-MS/MS方法可以准确地区分加工的松鼠,确保产品的真实性.

科学领域:

药物鉴定和分析化学
野生动物法医
保护科学

背景情况:

鱼的很有价值,但很难分类,尤其是在加工后.
传统方法缺乏准确认证所需的具体性和速度.
一种可靠的方法对于控制PS产品的质量和来源至关重要.

研究的目的:

开发一种可靠的分析方法来区分松鼠种.
为了快速识别PS及其加工形式.
支持甲产品的真实性和质量控制.

主要方法:

结合超高性能液体染色学-四极飞行时间质谱 (UPLC/Q-TOF-MS) 与主要成分分析 (PCA).
确定了七种特定物种的离子.
建立了一种液体染色体-双重质谱 (LC-MS/MS) 多重反应监测 (MRM) 方法.

主要成果:

通过UPLC/Q-TOF-MS和PCA方法,成功地将PS从三个物种中区分出来.
七个特定标记离子对各自的物种具有很高的特异性.
通过LC-MS/MS MRM方法实现了快速 (20分钟) 和准确的物种识别.
该方法在58个未知PS样本上得到了高可靠性验证.

结论:

一种新的LC-MS/MSMRM方法提供了有效的解决方案来识别松鼠的物种.
这种方法适用于原始和加工的松鼠.
这项研究提供了一种宝贵的工具,用于认证皮产品的真实性和质量.

关键词:

鱼的类品种认证特定物种的标记物

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