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在Th(IV) 应力下对RAW264.7细胞损伤机制的蛋白质组学分析
Lei Liu1, Panpan Guo2, Mingxia Zhang2
1Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China; School of Environment and Chemical Engineering, Anhui Vocational and Technical College, Hefei 230011, China.
Ecotoxicology and environmental safety
|August 20, 2025
概括
(IV) 暴露会通过降低活力和改变关键细胞功能来损害巨细胞. 这项研究揭示了.
科学领域:
- 环境毒理学环境毒理学
- 细胞生物学 细胞生物学
- 蛋白质组学是指蛋白质组学.
背景情况:
- (Th) 是一种天然存在的元素,具有潜在的人类健康风险.
- 了解对细胞机制的影响对于风险评估至关重要.
研究的目的:
- 为了研究 (IV) 对RAW264.7小鼠巨细胞的有毒作用.
- 为了阐明由暴露引起的潜在细胞损伤机制.
主要方法:
- 在RAW264.7细胞上进行了细胞活力测试.
- 评估了活性氧物种 (ROS) 和线粒体膜潜力 (MMP) 的变化.
- 利用蛋白质组学和西方污点分析来研究分子机制.
主要成果:
- (IV) 暴露使RAW264.7细胞活力以剂量依赖的方式降低.
- (IV) 诱导氧化应激 (增加ROS) 和线粒体功能障碍 (减少MMP).
- 蛋白质组学发现了核和运输相关蛋白质的变化,影响了细胞生长.
结论:
- (IV) 通过诱导氧化应激和线粒体损伤,对巨细胞有毒作用.
- 分子变化表明 (IV) 通过蛋白质表达变化抑制细胞生长.
- 这项研究提供了对中毒性机制的洞察力.
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