睡眠不足通过降低时钟基因Per2来加剧实验性结肠炎
Xuejun Jiang1, Pengcheng Wu2, Hui Chen1
1Institute of Molecular Rhythm and Metabolism, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
Biochemical pharmacology
|August 20, 2025
概括
睡眠不足会通过抑制PER2基因,破坏酸代谢并增加炎症,使肠道疾病 (IBD) 恶化. 这突显了昼夜节律与IBD严重程度之间的联系.
科学领域:
- 时间生物学
- 胃肠病学
- 分子生物学
背景情况:
- 肠道疾病 (IBD) 与昼夜节律的干扰有关.
- 睡眠不足已知会加剧结肠炎,但其潜在机制尚不清楚.
研究的目的:
- 研究PER2昼夜基因在睡眠不足引起的结肠炎恶化中的作用.
- 阐明睡眠不足,PER2和结肠炎之间的分子机制.
主要方法:
- 患有DSS诱导的大肠炎的小鼠每天都受到睡眠剥夺.
- 在结肠组织中分析了PER2的表达.
- 对患有大肠炎的Per2缺乏的小鼠进行了转录组分析.
- 使用CT-26细胞进行了体外实验.
主要成果:
- 在小鼠中,睡眠不足显著加剧了急性和慢性结肠炎.
- 在SD和结肠炎中,PER2表达被抑制,结合时效果更为明显.
- 缺乏Per2会增加对结肠炎和酸代谢的易感性.
- 在SD和Per2缺乏性结肠炎模型中观察到Ptgs2 (COX-2) 的升高和PGE2水平的升高.
- 在体外发现Per2通过BMAL1抑制COX-2转录.
结论:
- 睡眠不足抑制了结肠的PER2表达,导致COX-2介导的酸代谢失调.
- 这种干扰会释放促炎媒介,加剧结肠炎.
- PER2是睡眠不足导致大肠炎恶化的关键媒介.
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